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Titolo:
EPIDERMAL GROWTH-FACTOR RECEPTOR EXPRESSION IN SQUAMOUS-CELL LUNG CARCINOMAS - AN IMMUNOHISTOCHEMICAL AND GENE ANALYSIS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED MATERIAL
Autore:
GORGOULIS V; GIATROMANOLAKI A; KARAMERIS A; TSATSANIS C; ANINOS D; OZANNE B; VESLEMES M; JORDANOGLOU J; TRIGIDOU R; PAPASTAMATIOU H; SPANDIDOS DA;
Indirizzi:
JOHN RAGOU 10 GR-11524 ATHENS GREECE RED CROSS HOSP,DEPT PATHOL ATHENS GREECE NATL HELLEN RES FDN GR-5011 ATHENS GREECE ARMY GEN HOSP 401,DEPT PATHOL ATHENS GREECE BEATSON INST CANC RES GLASGOW G61 1BD SCOTLAND UNIV ATHENS,SOTIRIA HOSP,SCH MED,DEPT PULM ATHENS GREECE GEN HOSP ATHENS,DEPT CYTOL ATHENS GREECE SOTIRIA HOSP,DEPT PATHOL ATHENS GREECE UNIV CRETE,SCH MED IRAKLION GREECE
Titolo Testata:
Virchows Archiv. A. Pathological anatomy and histology
fascicolo: 4, volume: 423, anno: 1993,
pagine: 295 - 302
SICI:
0174-7398(1993)423:4<295:EGREIS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EGF RECEPTOR; TUMORS; INTERNALIZATION; AMPLIFICATION; LOCALIZATION; ANTIBODY; BINDING;
Keywords:
EPIDERMAL GROWTH FACTOR RECEPTOR; SQUAMOUS CELL LUNG CARCINOMA; SOUTHERN BLOT; DIFFERENTIAL POLYMERASE CHAIN REACTION; IMMUNOHISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
V. Gorgoulis et al., "EPIDERMAL GROWTH-FACTOR RECEPTOR EXPRESSION IN SQUAMOUS-CELL LUNG CARCINOMAS - AN IMMUNOHISTOCHEMICAL AND GENE ANALYSIS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED MATERIAL", Virchows Archiv. A. Pathological anatomy and histology, 423(4), 1993, pp. 295-302

Abstract

Epidermal growth factor (EGF) and its receptor (EGFr) constitute an important and well-characterized mitogenic system in various ectodermaltissues. We evaluated the expression of EGFr and examined possible EGFr gene alterations in 18 formalin-fixed, paraffin-embedded squamous cell lung carcinomas (SCLC) by an immunohistochemical assay, Southern blotting and differential polymerase chain reaction (DPCR). The immunohistochemical study employing the F4 and EGF-R1 monoclonal antibodies, directed against the intra- and extra-cellular portion of the receptorrespectively, showed EGFr over-expression in 89% of the SCLC cases examined. All cases showed positive immunostaining for both antibodies, thus excluding the possibility of truncated receptors. In addition, analysis of the EGFr gene was carried out by Southern blotting and DPCR on paraffin extracted DNA from the same carcinoma cases. We found amplification of the EGFr gene in 5/18 (27%) SCLCs. All 5 positive cases showed EGFr over-expression, suggesting a possible correlation between the presence of EGFr gene amplification and over-expression of receptor protein. No correlation was observed among EGFr staining, EGFr gene amplification and differentiation of carcinomas. In addition, Southernblot analysis with HER-A2, a probe which hybridizes a sequence of thereceptor's intracellular domain, revealed three novel EcoRI restriction fragment patterns. We suggest that these patterns correspond to EcoRI polymorphic sites of the receptor's tyrosine kinase domain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:53:22