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Titolo:
TREATMENT WITH RECOMBINANT GRANULOCYTE-COLONY-STIMULATING FACTOR (FILGRASTIN(TM)) STIMULATES NEUTROPHILS AND TISSUE MACROPHAGES AND INDUCESAN EFFECTIVE NONSPECIFIC RESPONSE AGAINST MYCOBACTERIUM-AVIUM IN MICE
Autore:
BERMUDEZ LE; PETROFSKY M; STEVENS P;
Indirizzi:
CALIF PACIFIC MED CTR,RES INST,KUZELL INST ARTHRITIS & INFECT DIS,2200 WEBSTER ST,SUITE 305 SAN FRANCISCO CA 94115 AMGEN CORP THOUSAND OAKS CA 91320
Titolo Testata:
Immunology
fascicolo: 3, volume: 94, anno: 1998,
pagine: 297 - 303
SICI:
0019-2805(1998)94:3<297:TWRGF(>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR G-CSF; TUMOR NECROSIS FACTOR; COMPLEX INFECTION; ENHANCED RESISTANCE; MURINE MACROPHAGES; MOUSE RESISTANCE; INHIBIT GROWTH; KILLER-CELLS; EXPRESSION; IL-10;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
L.E. Bermudez et al., "TREATMENT WITH RECOMBINANT GRANULOCYTE-COLONY-STIMULATING FACTOR (FILGRASTIN(TM)) STIMULATES NEUTROPHILS AND TISSUE MACROPHAGES AND INDUCESAN EFFECTIVE NONSPECIFIC RESPONSE AGAINST MYCOBACTERIUM-AVIUM IN MICE", Immunology, 94(3), 1998, pp. 297-303

Abstract

A role of neutrophils in the host response against Mycobacterium avium (MAC) has recently been suggested. To investigate this matter further, Eve determined the effect of granulocyte colony-stimulating factor (G-CSF) on the outcome of MAC infection in mice. C57BL/6 bg(+)/bg(-) black mice were intravenously infected with 1x10(7) MAC and then divided into four experimental groups to receive G-CSF as follows: (i) 10 mug/kg/day; (ii) 50 mu g/kg/day; (iii) 100 mu g/kg/day; (iv) placebo control. Mice were killed at 2 and 4 weeks of treatment to determine thebacterial load of liver and spleen. Treatment with G-CSF at both 10 and 50 mu g/kg/day doses significantly decreased the number of viable bacteria in liver and spleen after 2 weeks (approximate to 70.5% and 69.0%, respectively), and after 4 weeks (approximate to 53% and 52%, respectively, P<0.05 compared with placebo control), Treatment with 100 mu g/kg/day did not result in decrease of bacterial colony-forming units in the liver and spleen after 4 weeks. Administration of G-CSF induced interleukin-10 (IL-10) and IL-12 production by splenocytes. To examine if the protective effect of G-CSF was accompanied by the activation of phagocytic cells, blood neutrophils and splenic macrophages were purified from mice receiving G-CSF and their ability to kill MAC was examined ex vivo. Neutrophils and macrophages from G-CSF-treated mice were able to inhibit the growth of or to kill MAC ex vivo, while phagocytic cells from untreated control mice had no anti-MAC effect. These results suggest that activation of neutrophils appears to induce an effective non-specific host defence against MAC, and further studies should aim for better understanding of the mechanisms of protection.

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Documento generato il 01/10/20 alle ore 06:32:09