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Titolo:
APOLIPOPROTEIN(A) SYNTHESIS AND SECRETION FROM HEPATOMA-CELLS IS COUPLED TO TRIGLYCERIDE SYNTHESIS AND SECRETION
Autore:
NASSIR F; BONEN DK; DAVIDSON NO;
Indirizzi:
UNIV CHICAGO,DEPT MED,5841 S MARYLAND AVE,MC 4076 CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637
Titolo Testata:
The Journal of biological chemistry
fascicolo: 28, volume: 273, anno: 1998,
pagine: 17793 - 17800
SICI:
0021-9258(1998)273:28<17793:ASASFH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA LIPOPROTEIN(A) CONCENTRATIONS; APO-B SECRETION; HEP G2 CELLS; INTRACELLULAR DEGRADATION; BABOON HEPATOCYTES; TRANSLOCATION; PROTEIN; GENE; MUTAGENESIS; PHENOTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
F. Nassir et al., "APOLIPOPROTEIN(A) SYNTHESIS AND SECRETION FROM HEPATOMA-CELLS IS COUPLED TO TRIGLYCERIDE SYNTHESIS AND SECRETION", The Journal of biological chemistry, 273(28), 1998, pp. 17793-17800

Abstract

Apolipoprotein(a) (apo(a)) is synthesized and secreted from liver cells and represents one of the two major protein components of the atherogenic lipoprotein, Lp(a), Little is known, however, of the factors that regulate the secretion of this protein. We have undertaken an analysis of the response to oleate supplementation in stable clones of HepG2 and McA-RH7777 cells express ing either a 6 K-IV or 17 K-IV isoform of apo(a), These cell lines were examined by pulse chase analysis and each demonstrated an increase (range 2-6-fold) in apo(a) secretion following supplementation with 0.8 mM oleate, Microsomal membranes, prepared from HepG2 cells expressing a 6 K-TV apo(a) isoform, demonstrated that oleate supplementation increased the apparent protection of apo(a) from protease digestion, suggesting that alterations in the translocation efficiency of apo(a) may accompany the addition of oleate, Cellsincubated with brefeldin A demonstrated increased recovery of the precursor form of apo(a) with oleate supplementation, suggesting that alterations in post-translational degradation may also contribute to the observed in crease in apo(a) secretion following oleate addition. To further characterize the oleate-dependent increase in apo(a) secretion,cells were incubated with an inhibitor of the microsomal triglyceridetransfer protein. These experiments demonstrated a dose dependent decrease in apo(a) secretion from both cell lines. Furthermore, addition of either the microsomal triglyceride transfer protein inhibitor or triacsin C, an inhibitor of acyl-CoA synthase, completely abrogated the oleate-dependent increase in apo(a) secretion. Taken together, these data provide evidence that apo(a) secretion from hepatoma cells may be linked to elements of cellular triglyceride assembly and secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 10:24:06