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Titolo:
AUTOMATED-DETERMINATION OF CLOMIPRAMINE AND ITS MAJOR METABOLITES IN HUMAN AND RAT SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ONLINE COLUMN-SWITCHING
Autore:
WEIGMANN H; HARTTER S; HIEMKE C;
Indirizzi:
UNIV MAINZ,DEPT PSYCHIAT,UNTERE ZAHLBACHER STR 8 D-55131 MAINZ GERMANY UNIV MAINZ,DEPT PSYCHIAT D-55131 MAINZ GERMANY
Titolo Testata:
Journal of chromatography B. Biomedical sciences and applications
fascicolo: 1-2, volume: 710, anno: 1998,
pagine: 227 - 233
SICI:
0378-4347(1998)710:1-2<227:AOCAIM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-PLASMA; HYDROXYLATED METABOLITES; AMITRIPTYLINE; ANTIDEPRESSANTS; SEPARATION; PHASE; DRUGS; URINE; HPLC;
Keywords:
CLOMIPRAMINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
H. Weigmann et al., "AUTOMATED-DETERMINATION OF CLOMIPRAMINE AND ITS MAJOR METABOLITES IN HUMAN AND RAT SERUM BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ONLINE COLUMN-SWITCHING", Journal of chromatography B. Biomedical sciences and applications, 710(1-2), 1998, pp. 227-233

Abstract

A fully automated method including column-switching and isocratic high-performance liquid chromatography (HPLC) was developed for simultaneous determination of the tricyclic antidepressant clomipramine and itsmetabolites demethylclomipramine, 2-, 8-, and 10-hydroxyclomipramine,2-, and 8-hydroxydemethylclomipramine and didemethylclomipramine in serum. After serum injection into the HPLC system and on-line sample clean-up on a clean-up column (Hypersil CN; 10X4.6 mm) by an eluent consisting of 35% acetonitrile and 65% deionized water, the chromatographic separation was performed on an analytical column (LiChrospher CN; 250x4.6 mm I.D.) by an eluent consisting of 38% acetonitrile and 62% aqueous sodium perchlorate (0.02 M, pH 2.5). The UV detector was set at 260 nm. The limit of quantification was about 15 ng/ml for all analytes. The coefficients of variation ranged between 3 and 12% with recoveryrates between 64 and 110%. Linear regression analyses revealed coefficients of correlation between 0.98 and 0.99. The method could be applied to therapeutic drug monitoring as well as metabolism studies in manand rat. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:03:47