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Titolo:
METHOD FOR THE DETERMINATION OF 5,5-DIPHENYLBARBITURIC ACID AND ITS SEPARATION FROM 1,3-DIMETHOXYMETHYL-5,5-DIPHENYLBARBITURIC ACID IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
THACKER DL; ABERNETHY DR; RAINES A; FLOCKHART DA;
Indirizzi:
GEORGETOWN UNIV,MED CTR,DEPT PHARMACOL,DIV CLIN PHARMACOL,3900 RESERVOIR RD NW WASHINGTON DC 20007 GEORGETOWN UNIV,MED CTR,DEPT PHARMACOL,DIV CLIN PHARMACOL WASHINGTON DC 20007 GEORGETOWN UNIV,MED CTR,DEPT MED,DIV CLIN PHARMACOL WASHINGTON DC 20007
Titolo Testata:
Journal of chromatography B. Biomedical sciences and applications
fascicolo: 1-2, volume: 710, anno: 1998,
pagine: 149 - 155
SICI:
0378-4347(1998)710:1-2<149:MFTDO5>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Keywords:
5,5-DIPHENYLBARBITURIC ACID; 1,3-DIMETHOXYMETHYL-5,5-DIPHENYLBARBITURIC ACID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
D.L. Thacker et al., "METHOD FOR THE DETERMINATION OF 5,5-DIPHENYLBARBITURIC ACID AND ITS SEPARATION FROM 1,3-DIMETHOXYMETHYL-5,5-DIPHENYLBARBITURIC ACID IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of chromatography B. Biomedical sciences and applications, 710(1-2), 1998, pp. 149-155

Abstract

A method is described for the measurement of 5,5-diphenylbarbituric acid in plasma using high-performance liquid chromatography with UV detection. Briefly, the compounds are separated on a C-18 reversed-phase column using a mobile phase of 50 mM sodium acetate (pH 4.5) and methanol. The flow-rate is 1.0 ml/min and 25 mu l are injected and detectedat 215 nm. The method is specific and sensitive in the range of concentrations tested, with a limit of quantification of 0.25 mu g/ml. The calibration curves are linear for concentrations between 0.25 and 10 mu g/ml. Intra-day and inter-day coefficients of variation are less than 8.5 and 10.5%, respectively, over the linear range. Intra-day and inter-day bias are less than 7.0 and 8.0%, respectively. A pharmacokinetic study conducted in male Beagle dogs administered 10 mg/kg of 1,3-dimethoxy methyl-5,5-diphenylbarbituric acid or 8 mg/kg of 5,5-diphenylbarbituric acid intravenously demonstrates the utility of this method. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:44:46