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Titolo:
EXPRESSION AND PRESENCE OF SEPTAL NEUROKININ-2 RECEPTORS CONTROLLING HIPPOCAMPAL ACETYLCHOLINE-RELEASE DURING SENSORY STIMULATION IN RAT
Autore:
STEINBERG R; MARCO N; VOUTSINOS B; BENSAID M; RODIER D; SOUILHAC J; ALONSO R; OURYDONAT F; LEFUR G; SOUBRIE P;
Indirizzi:
371 RUE PROF BLAYAC F-34184 MONTPELLIER 4 FRANCE
Titolo Testata:
European journal of neuroscience
fascicolo: 7, volume: 10, anno: 1998,
pagine: 2337 - 2345
SICI:
0953-816X(1998)10:7<2337:EAPOSN>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DISTINCT PRESYNAPTIC CONTROL; NK3 TACHYKININ RECEPTORS; MATRIX-ENRICHED AREAS; BINDING-SITES; MEDIAL SEPTUM; CHOLINERGIC NEURONS; SELECTIVE AGONISTS; DOPAMINE RELEASE; MESSENGER-RNA; K RECEPTOR;
Keywords:
MICRODIALYSIS; NEUROKININ A; SEPTOHIPPOCAMPAL PATHWAY; SR144190; SR48968;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
R. Steinberg et al., "EXPRESSION AND PRESENCE OF SEPTAL NEUROKININ-2 RECEPTORS CONTROLLING HIPPOCAMPAL ACETYLCHOLINE-RELEASE DURING SENSORY STIMULATION IN RAT", European journal of neuroscience, 10(7), 1998, pp. 2337-2345

Abstract

We examined the expression and presence of NK2 receptors in the septal area of rat brain, and investigated their functional role in the regulation of the septohippocampal cholinergic system. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we showed the presence of NK2 receptor mRNA expression in the septal area, and detected septal NK2 binding sites by using a fluorescent-tagged neurokinin A(NKA) derivative. In vivo microdialysis was employed to explore the functional role of NK2 receptors in the release of hippocampal acetylcholine evoked by tactile stimulation in freely moving rats. Two sessions of stroking of the neck and back of the rat for 30 min, at 90 min intervals, produced a marked and reproducible increase in hippocampal acetylcholine release. This effect was dose-dependently prevented by intraperitoneal administration of the two selective non-peptide tachykinin NK2 receptor antagonists SR144190 (0.03-0.3 mg/kg, i.p.) and SR48968(0.3 and 1 mg/kg, i.p.), but not by the inactive enantiomer of SR48968 (SR48965, 1 mg/kg) nor by the two non-peptide NK1 receptor antagonists SR140333 (3 mg/kg, i.p.) and GR205171 (1 mg/kg, i.p.). Furthermore,the intraseptal application of SR144190 (10(-8) M) reduced the sensory response. Finally, intraseptal perfusion of neurokinin A (0.01-10 muM) in anaesthetized rats produced a concentration-dependent increase in hippocampal acetylcholine release. The response to neurokinin A (0.1 mu M) was prevented by SR144190 (0.03-0.3 mg/kg, i.p.) and SR48968 (0.3-1 mg/kg, i.p.). In conclusion, this study provides direct evidencefor the role of endogenous NKA/substance P, through the activation ofNK2 receptors, in regulating the septohippocampal cholinergic function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:38:47