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Titolo:
INTESTINAL UPTAKE OF LIPOVITELLIN FROM BRINE SHRIMP (ARTEMIA-FRANCISCANA) BY LARVAL INLAND SILVERSIDES (MENIDIA-BERYLLINA) AND STRIPED BASS(MORONE-SAXATILIS)
Autore:
KISHIDA M; JOHANNING KM; BENGTSON DA; SPECKER JL;
Indirizzi:
UNIV RHODE ISL,GRAD SCH OCEANOG,BOX 14,S FERRY RD NARRAGANSETT RI 02882 UNIV RHODE ISL,GRAD SCH OCEANOG NARRAGANSETT RI 02882 UNIV RHODE ISL,DEPT FISHERIES ANIM & VET SCI KINGSTON RI 02881
Titolo Testata:
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
fascicolo: 1, volume: 119, anno: 1998,
pagine: 415 - 421
SICI:
1095-6433(1998)119:1<415:IUOLFB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TILAPIA OREOCHROMIS-MOSSAMBICUS; INTRACELLULAR DIGESTION; HORSERADISH-PEROXIDASE; VITELLOGENIN; PROTEINS; EMBRYOS; LIPASE; CELLS; FISH;
Keywords:
FISH LARVAE; INTESTINE; NUTRIENT ABSORPTION; DEVELOPMENT; LIPOPROTEIN UPTAKE; IMMUNOLOCALIZATION; ARTEMIA LIPOVITELLIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
M. Kishida et al., "INTESTINAL UPTAKE OF LIPOVITELLIN FROM BRINE SHRIMP (ARTEMIA-FRANCISCANA) BY LARVAL INLAND SILVERSIDES (MENIDIA-BERYLLINA) AND STRIPED BASS(MORONE-SAXATILIS)", Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 119(1), 1998, pp. 415-421

Abstract

Intestinal uptake of lipovitellin (LV) from brine shrimp (Artemia franciscana) in larval inland silversides (Menidia beryllina) and stripedbass (Morone saxatilis) was described using immunocytochemistry. Polyclonal antisera were raised against two subunits of LV (LV68 and LV190). When tested by immunocytochemistry, anti-LV68 showed cross-reactivity with some of the pancreatic cells especially in inland silversides. Therefore anti-LV190 was used to localize immunoreactive LV. Inland silversides at 14 days after hatching were led Artemia nauplii and thensampled 4, 8, 12 hr after feeding. Similar experiments were carried out by using striped bass at 5 days and 15 days of age. They were sampled at 2, 4, 8, and 12 hr after feeding. Anterior enterocytes showed noevidence of uptake; however, the brush border of the cells of inland silversides reacted with the antiserum. Posterior enterocytes took up the LV and/or, possibly, their immunoreactive breakdown products. The pattern of uptake included accumulation in supranuclear vacuoles and digestion in supranuclear vacuoles, as suggested by the decay of the immunoreactivity over time. Thus, the posterior intestine of these larval fishes is the site of uptake and digestion of LV, an important nutritive component in the food of many larval fishes; this supports earlier findings using non-nutritive marker proteins. (C) 1998 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 13:19:08