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Titolo:
PHENOBARBITAL PREVENTS THE INHIBITORY EFFECTS OF TUMOR-NECROSIS-FACTOR ON GLUTATHIONE-S-TRANSFERASE-MU IN PRIMARY CULTURE RAT HEPATOCYTES
Autore:
MASSAADMASSADE L; CHOUAIB S; GOUYETTE A;
Indirizzi:
INSERM,U490,MOL TOXICOL LAB,45 RUE ST PERES F-75270 PARIS 06 FRANCE INST GUSTAVE ROUSSY,INSERM,CJF 9411 F-94805 VILLEJUIF FRANCE INST GUSTAVE ROUSSY,DEPT PHARMACOTOXICOL & PHARMACOGENET,CNRS,URA F-94805 VILLEJUIF FRANCE
Titolo Testata:
Anticancer research
fascicolo: 3A, volume: 18, anno: 1998,
pagine: 1833 - 1838
SICI:
0250-7005(1998)18:3A<1833:PPTIEO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; LIVER; EXPRESSION; INDUCTION; PROTEINS; ENZYMES; CELLS; INTERLEUKIN-1-BETA; MECHANISMS; CACHECTIN;
Keywords:
TUMOR NECROSIS FACTOR; PHENOBARBITAL; GLUTATHIONE; S-TRANSFERASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
L. Massaadmassade et al., "PHENOBARBITAL PREVENTS THE INHIBITORY EFFECTS OF TUMOR-NECROSIS-FACTOR ON GLUTATHIONE-S-TRANSFERASE-MU IN PRIMARY CULTURE RAT HEPATOCYTES", Anticancer research, 18(3A), 1998, pp. 1833-1838

Abstract

During inflammation and infection, overexpression of the tumor necrosis factor (TNF) is associated with changes in cytochromes P-450 levelsin rat and human hepatocytes. The aim of this study was to investigate the effect of TNF on the expression of the glutathione-S-transferases (GSTs) in rat hepatocytes. TNF was added in vitro alone or simultaneously with phenobarbital (PB) into hepatocytes in primary culture or in vivo, before TNF, injected directly to rats. GST activity was assayed by spectrophotometry; protein GSTs alpha, mu and pi were evaluated by immunoblotting. When TNF was added alone to rat hepatocytes in vitro, fetal GST activity and GST alpha levels were not affected, while GSTmu protein levels significantly decreased by 35%. GST pi protein was undetectable in hepatocytes whether treated or not with TNF. When PB was administered in vitro simultaneously to rat hepatocytes with TNF, the decrease observed for GST mu subunit was suppressed while total GSTactivity and GST alpha content were not affected When hepatocytes were,treated with TNF after PB given in vivo directly to the rat by ip injection, GST activity and GSTs subunits were induced by PB, while TNF did not exert any effect These results indicate that TNF has an inhibitory effect on GST mu and PB abrogates this effect in primary culturedvat hepatocytes. Then, PB could prevent some TNF toxic effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:29:20