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Titolo:
ADENOASSOCIATED VIRUS VECTORS CAN BE EFFICIENTLY PRODUCED WITHOUT HELPER VIRUS
Autore:
MATSUSHITA T; ELLIGER S; ELLIGER C; PODSAKOFF G; VILLARREAL L; KURTZMAN GJ; IWAKI Y; COLOSI P;
Indirizzi:
1201 HARBOR BAY PKWY,SUITE 1000 ALAMEDA CA 94502 AVIGEN INC ALAMEDA CA 00000 UNIV CALIF IRVINE,DEPT MOL BIOL & BIOCHEM IRVINE CA 92717 UNIV SO CALIF,SCH MED,DEPT UROL LOS ANGELES CA 90033
Titolo Testata:
Gene therapy
fascicolo: 7, volume: 5, anno: 1998,
pagine: 938 - 945
SICI:
0969-7128(1998)5:7<938:AVVCBE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING PROTEIN; PRODUCTIVELY INFECTED-CELLS; HERPES-SIMPLEX VIRUS; EARLY REGION 4; ADENOASSOCIATED VIRUS; MESSENGER-RNAS; GENE-EXPRESSION; VAI RNA; REPLICATION; TYPE-5;
Keywords:
AAV VECTOR PRODUCTION; AAV HELPER GENES; ADENOVIRUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
T. Matsushita et al., "ADENOASSOCIATED VIRUS VECTORS CAN BE EFFICIENTLY PRODUCED WITHOUT HELPER VIRUS", Gene therapy, 5(7), 1998, pp. 938-945

Abstract

The purpose of this work was to develop an efficient method for the production of adeno-associated virus (AAV) vectors in the absence of helper virus. The adenovirus regions that mediate AAV vector replicationwere identified and assembled into a helper plasmid. These included the VA, E2A and E4 regions. When this helper plasmid was cotransfected into 293 cells, along with plasmids encoding the AAV vector, and rep and cap genes, AAV vector was produced as efficiently as when using adenovirus infection as a source of help. CMV-driven constructs expressing the E4orf6 and the 72-M-r, E2A proteins were able to functionally replace the E4 and E2A regions, respectively. Therefore the minimum set of genes required to produce AAV helper activity equivalent to that provided by adenovirus infection consists of, or is a subset of, the following genes: the E4orf6 gene, the 72-M-r, E2A protein gene, the VA RNA genes and the E1 region. AAV vector preparations made with adenovirus and by the helper virus-free method were essentially indistinguishable with respect to particle density, particle to infectivity ratio, capsimer ratio and efficiency of muscle transduction in vivo. Only AAV vector preparations made by the helper virus-free method were not reactive with anti-adenovirus sera.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 11:11:54