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Titolo:
ASSESSMENT OF RESISTANCE INDUCTION TO CYTOSINE-ARABINOSIDE FOLLOWING TRANSFER AND OVEREXPRESSION OF THE DEOXYCYTIDYLATE DEAMINASE GENE IN-VITRO
Autore:
SCHRODER JK; SEIDELMANN M; KIRCH HC; SEEBER S; SCHUTTE J;
Indirizzi:
UNIV ESSEN GESAMTHSCH KLINIKUM,INNERE KLIN & POLIKLIN TUMORFORSCH,W DEUTSCH TUMORZENTRUM D-45122 ESSEN GERMANY UNIV ESSEN GESAMTHSCH,SCH MED,W GERMAN CANC CTR,DEPT INTERNAL MED CANC RES ESSEN GERMANY UNIV ESSEN GESAMTHSCH,SCH MED,W GERMAN CANC CTR,INST MOL BIOL CANC RES ESSEN GERMANY
Titolo Testata:
Leukemia research
fascicolo: 7, volume: 22, anno: 1998,
pagine: 619 - 624
SICI:
0145-2126(1998)22:7<619:AORITC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYELOID-LEUKEMIA; BONE-MARROW; HEMATOPOIETIC-CELLS; RETROVIRAL TRANSFER; CYTIDINE DEAMINASE; CONFERS RESISTANCE; HUMAN MDR1; EXPRESSION; TRANSDUCTION; ORGANIZATION;
Keywords:
MYELOID LEUKEMIA; CYTOSINE ARABINOSIDE; DEOXYCYTIDYLATE DEAMINASE; CYTIDINE DEAMINASE; DRUG RESISTANCE; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
J.K. Schroder et al., "ASSESSMENT OF RESISTANCE INDUCTION TO CYTOSINE-ARABINOSIDE FOLLOWING TRANSFER AND OVEREXPRESSION OF THE DEOXYCYTIDYLATE DEAMINASE GENE IN-VITRO", Leukemia research, 22(7), 1998, pp. 619-624

Abstract

Recent attempts to protect hematopoietic progenitor cells from cytarabine (ara-C)-induced toxicity by transfer of the cytidine deaminase (CDD) gene resulted in efficient in vitro inducibility of ara-C resistance. Another enzyme involved in intracellular ara-CTP inactivation is the deoxycytidylate deaminase (dCMPD). We therefore transfected the human dCMPD cDNA gene into murine fibroblasts and investigated the relationship of forced dCMPD expression and resistance induction to ara-C. Several cell lines were established which demonstrated a 1.7-3.5-fold increase in cellular dCMPD activity and an up to 2-fold increase in theIC50 value of ara-C. However, increases in dCMPD activities did not show a positive linear correlation with the induction of ara-C resistance. In addition, CD34+ hematopoietic progenitor cells revealed the highest endogenous dCMPD enzyme levels among different human hematopoietic cells. Thus, despite the documented role for dCMPD in ara-CTP inactivation of certain cell types, these results suggest that the dCMPD gene may prove less useful than the CDD gene as a therapeutic target in attempts to attenuate ara-C-induced bone marrow toxicity. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/10/20 alle ore 09:15:00