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Titolo:
ONTOGENY-ASSOCIATED CHANGES IN THE CYTOKINE RESPONSES OF PRIMITIVE HUMAN HEMATOPOIETIC-CELLS
Autore:
ZANDSTRA PW; CONNEALLY E; PIRET JM; EAVES CJ;
Indirizzi:
BRITISH COLUMBIA CANC AGCY,TERRY FOX LAB,601 W 10TH AVE VANCOUVER BC V5Z 1L3 CANADA BRITISH COLUMBIA CANC AGCY,TERRY FOX LAB VANCOUVER BC V5Z 1L3 CANADA UNIV BRITISH COLUMBIA,DEPT CHEM ENGN VANCOUVER BC V6T 1W5 CANADA UNIV BRITISH COLUMBIA,DEPT EXPT PATHOL & LAB MED VANCOUVER BC V6T 1W5CANADA UNIV BRITISH COLUMBIA,DEPT MED GENET & MED VANCOUVER BC V6T 1W5 CANADA UNIV BRITISH COLUMBIA,BIOTECHNOL LAB VANCOUVER BC V6T 1W5 CANADA
Titolo Testata:
British Journal of Haematology
fascicolo: 4, volume: 101, anno: 1998,
pagine: 770 - 778
SICI:
0007-1048(1998)101:4<770:OCITCR>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; UMBILICAL-CORD BLOOD; ADULT BONE-MARROW; EX-VIVO EXPANSION; PROGENITOR CELLS; FETAL LIVER; CD34(+) CELLS; IN-VITRO; DEVELOPMENTAL SWITCH; TRANSPLANTATION;
Keywords:
ONTOGENY; CORD BLOOD; EXPANSION; STEM CELLS; CYTOKINES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
P.W. Zandstra et al., "ONTOGENY-ASSOCIATED CHANGES IN THE CYTOKINE RESPONSES OF PRIMITIVE HUMAN HEMATOPOIETIC-CELLS", British Journal of Haematology, 101(4), 1998, pp. 770-778

Abstract

Time course studies revealed that the combina tion of Flt-3 ligand (FL), Steel factor (SF) and interleukin-3 (IL-3) did not elicit as largean amplification of the long-term culture-initiating cell (LTC-IC) population in serum-free cultures of CD34(+)CD38(-) cord blood (CB) cells as was obtained in similar cultures of adult human CD34(+)-CD38(-) bone marrow (BM) cells (4- v 90-fold maximum increases), even though both total and colony-forming cell (CFC) numbers initially increased more rapidly in CB cultures. Multifactorial analysis of the short-term (10d) effects of different cytokines identified FL and IL-6 in combination with the soluble IL-6 receptor (sIL-6R) as most important for expanding the CB LTC-IC population. In contrast, their counterparts in adult BM were most effectively stimulated by FL, SF and IL-3. For rapid generation of increased numbers of CFC, SF with either FL or IL-6/sIL-6Rwere found to be the most important contributors in cultures of CD34()CD38(-) CB cells, whereas, in analogous BM cultures, IL-6/sIL-6R andTPO tin addition to FL, SF and IL-3) were required. These findings reinforce the principle of altered cytokine responsiveness as a hallmarkof early haemopoietic cell differentiation and demonstrate how cytokine requirements may change during human ontogeny Identification of conditions for optimizing the expansion of different subsets of primitiveCB cells has additional important implications for clinical transplantation and gene transfer.

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Documento generato il 26/09/20 alle ore 11:11:16