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Titolo:
STEM-CELL FACTOR INDUCES PROLIFERATION AND DIFFERENTIATION OF FETAL PROGENITOR CELLS IN THE MOUSE
Autore:
KURATA H; MANCINI GC; ALESPEITI G; MIGLIACCIO AR; MIGLIACCIO G;
Indirizzi:
IST SUPER SANITA,LAB BIOL CELLULARE,VIALE REGINA ELENA 299 I-00161 ROME ITALY IST SUPER SANITA,LAB BIOL CELLULARE I-00161 ROME ITALY NEW YORK BLOOD CTR,LINDSLEY F KIMBALL RES INST,HEMATOPOIET GROWTH FACTORS LAB NEW YORK NY 10021 NIIGATA UNIV,SCH MED,DEPT OBSTET & GYNAECOL NIIGATA JAPAN
Titolo Testata:
British Journal of Haematology
fascicolo: 4, volume: 101, anno: 1998,
pagine: 676 - 687
SICI:
0007-1048(1998)101:4<676:SFIPAD>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-FORMING CELLS; ADULT BONE-MARROW; IN-VITRO; YOLK-SAC; STIMULATING FACTORS; HEMATOPOIETIC PROGENITORS; ERYTHROPOIETIN RECEPTOR; CFU-E; BFU-E; LIVER;
Keywords:
MURINE ONTOGENY; FETAL CELL PURIFICATION; STEM CELL FACTOR; GROWTH FACTOR RESPONSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
H. Kurata et al., "STEM-CELL FACTOR INDUCES PROLIFERATION AND DIFFERENTIATION OF FETAL PROGENITOR CELLS IN THE MOUSE", British Journal of Haematology, 101(4), 1998, pp. 676-687

Abstract

We have investigated the kinetics of the amplification of the progenitor cell compartments (CFC)in haemopoietic organs during murine ontogenesis and compared the growth requirements of fetal and adult CFC. Twohaemopoietic phases were recognized in the fetal liver (FL): an exponential growth phase, from 11.5 to 15.5 d post conception (p.c.), during which the mean number of nucleated cells and of CFC in the FL increased from 4.9x10(5) to 7.0x10(7) and from 4.5x10(3) to 27x10(5) respectively, and a recessive phase after 15.5d p.c., during which the CFC number in the FL gradually decreased, although some CFC were still detectable in the liver after birth. In serum-deprived cultures, FL and adult marrow (AM) CFC had similar responses to GM-CSF and did not respondto G-CSF or IL-3. In contrast, FL, but not AM, erythroid colonies grew Epo-independently whereas SCF alone induced formation of maximal numbers of erythroid bursts from FL, but not from AM cells. The proliferative and differentiative effect of SCF alone on fetal cells was confirmed in serum-deprived cultures of purified early progenitor cells isolated by cell sorting on the basis of multiple parameters from FL and AM light-density cells. In culture of purified FL cells, SCF alone induced a similar amplification of total cells (maximal amplification at day 12: 800-300-fold) and total CFC (11-38-fold of maximal amplification at day 6) to the combination of SCF plus IL-3 (1300-800-fold amplification of total cells and 31-88-fold amplification of CFC). In contrast, SCF alone allowed only survival of purified AM early progenitor cells. Therefore FL early progenitor cells have an intrinsic higher potential than their adult counterpart to respond to SCF, confirming the potent role of this growth factor in the development of the murine haemopoietic system.

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Documento generato il 26/09/20 alle ore 12:11:56