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Titolo:
MATURATIONAL CHANGES IN RABBIT RENAL BRUSH-BORDER MEMBRANE VESICLE OSMOTIC WATER PERMEABILITY
Autore:
QUIGLEY R; HARKINS EW; THOMAS PJ; BAUM M;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT PEDIAT DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT PHYSIOL DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED DALLAS TX 75235
Titolo Testata:
The journal of membrane biology
fascicolo: 2, volume: 164, anno: 1998,
pagine: 177 - 185
SICI:
0022-2631(1998)164:2<177:MCIRRB>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; RAT; TRANSPORT; KIDNEY; FLUIDITY; CHANNEL; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
R. Quigley et al., "MATURATIONAL CHANGES IN RABBIT RENAL BRUSH-BORDER MEMBRANE VESICLE OSMOTIC WATER PERMEABILITY", The journal of membrane biology, 164(2), 1998, pp. 177-185

Abstract

We have recently shown that the osmotic water permeability (P-f) of proximal tubules from neonatal rabbits is higher than that of adults (AJP 271:F871-F876, 1996). The developmental change in P-f could be due to differences in one or more of the components in the path for transepithelial water transport. The present study examined developmental changes in water transport characteristics of the proximal tubule apicalmembrane by determining P-f and aquaporin 1 (AQP1) expression in neonatal (10-14 days old) and adult rabbit renal brush border membrane vesicles (BBMV). AQP1 abundance in the adult BBMV was higher than the neonatal BBMV. At 25 degrees C the P-f of neonatal BBMV was found to be significantly lower than the adult BBMV at osmotic gradients from 50 to250 mOsm/kg water. The activation energy for osmotic water movement was higher in the neonatal BBMV than the adult BBMV (9.19 +/- 0.37 vs. 5.09 +/- 0.57 kcal . deg(-1) . mol(-1), P < 0.005). Osmotic water movement in neonatal BBMV was inhibited 17.9 +/- 1.3% by 1 mM HgCl2 compared to 34.3 +/- 3.8% in the adult BBMV (P < 0.005). These data are consistent with a significantly greater fraction of water traversing the apical membrane lipid bilayer in proximal tubules of neonates than adults. The lower P-f of the neonatal BBMV indicates that the apical membrane is not respon sible for the higher transepithelial P-f in the neonatal proximal tubule.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:39:34