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Titolo:
CLONING AND CHARACTERIZATION OF A NEW SWINE MHC (SLA) CLASS-II DQB ALLELE
Autore:
HOSOKAWA T; TANIOKA Y; TANIGAWA M; MATSUMOTO Y; ONODERA T; MATSUMOTO Y;
Indirizzi:
UNIV TOKYO,SCH AGR & LIFE SCI,DEPT MOL IMMUNOL,BUNKYO KU,1-1-1 YAYOI TOKYO 1138657 JAPAN UNIV TOKYO,SCH AGR & LIFE SCI,DEPT MOL IMMUNOL,BUNKYO KU TOKYO 1138657 JAPAN CENT INST EXPT ANIM,MIYAMAE KU KAWASAKI KANAGAWA 216000 JAPAN SLA RES INC SUWA NAGANO 3920016 JAPAN
Titolo Testata:
Journal of veterinary medical science
fascicolo: 6, volume: 60, anno: 1998,
pagine: 725 - 729
SICI:
0916-7250(1998)60:6<725:CACOAN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR HISTOCOMPATIBILITY COMPLEX; NUCLEOTIDE-SEQUENCES; INSITU HYBRIDIZATION; MINIATURE SWINE; GENES; CHROMOSOME-7; SUBSTITUTION; EXPRESSION; ASSIGNMENT; ANTIGEN;
Keywords:
MAJOR HISTOCOMPATIBILITY COMPLEX (MHC) CLASS II; PHYLOGENETIC TREE; SWINE LEUKOCYTE ANTIGEN (SLA); SLA-DQ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
T. Hosokawa et al., "CLONING AND CHARACTERIZATION OF A NEW SWINE MHC (SLA) CLASS-II DQB ALLELE", Journal of veterinary medical science, 60(6), 1998, pp. 725-729

Abstract

Major histocompatibility complex (MHC) of pigs is known as swine leukocyte antigen (SLA). The cDNA encoding a new allele of SLA class II DQbeta-chain was successfully isolated from a CSK miniature pig (derived from Gottingen strain) and characterized by sequence analyses. SLA-DQB cDNA fragment encoding beta(2)-domain was amplified by reverse transcriptase-polymerase chain reaction using the sequences preserved in avarious vertebrates as primers. Using non-radioisotope technique withthe PCR product as a probe, cDNA clone G01 was isolated from a spleencDNA library, and nucleotide sequence of this clone was determined. This clone encompassed a whole SLA-DQ beta-chain coding region, containing a total length of 1161 nucleotides with an open reading frame (ORF) of 786 nucleotides, 5' untranslated region of 15 nucleotides, and 3'untranslated region of 360 nucleotides ending with a canonical polyadenylation signal, followed by a poly A tail. Sequence comparisons of the ORF of this clone with those of known SLA-DQB genes confirmed that this clone is a new allele (SLA-DBBG01). Phylogenetic analysis of thenucleotide sequences of swine, human, and murine MHC class II genes indicated that SLA-DQB was more similar to HLA-DQB1 than H-2A beta. Comparison of the nucleotide and deduced amino acid sequences among SLA-DQB alleles showed that the SLA-DQ beta-chain polymorphism was found almost in beta(1)-domain which contains the antigenic peptide binding sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:20:12