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Titolo:
OVEREXPRESSION OF AN ARCHAEAL GERANYLGERANYL DIPHOSPHATE SYNTHASE IN ESCHERICHIA-COLI-CELLS
Autore:
OHTO C; NAKANE H; HEMMI H; OHNUMA S; OBATA S; NISHINO T;
Indirizzi:
TOYOTA MOTOR CO LTD,BIO RES LAB,1 TOYOTA CHO TOYOTA 4718572 JAPAN TOHOKU UNIV,DEPT BIOCHEM & ENGN,AOBA KU SENDAI MIYAGI 9808579 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 6, volume: 62, anno: 1998,
pagine: 1243 - 1246
SICI:
0916-8451(1998)62:6<1243:OOAAGD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PYROPHOSPHATE SYNTHASE; POLYPRENYL PYROPHOSPHATES; SACCHAROMYCES-CEREVISIAE; ENZYMATIC-HYDROLYSIS; NUCLEOTIDE-SEQUENCE; BIOSYNTHETIC GENE; PURIFICATION; CDNA; PRENYLTRANSFERASES; PRODUCTS;
Keywords:
SULFOLOBUS ACIDOCALDARIUS; PRENYL DIPHOSPHATE; FUSION PROTEIN; RECOMBINANT ENZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
C. Ohto et al., "OVEREXPRESSION OF AN ARCHAEAL GERANYLGERANYL DIPHOSPHATE SYNTHASE IN ESCHERICHIA-COLI-CELLS", Bioscience, biotechnology, and biochemistry, 62(6), 1998, pp. 1243-1246

Abstract

An archaeal geranylgeranyl diphosphate synthase was overexpressed in Escherichia coli cells as fusion proteins. These fusion proteins retained their thermostability and had higher specific activity than did a partially purified native enzyme Previously reported. We purified 24.3mg of MBP (maltose-binding protein)-fusion protein and 5.4 mg of GST (glutathione S-transferase)-fusion protein from a one-liter culture ofE. coli. The MBP-fusion proteins existed in dimer, tetramer, octamer,or dodecamer form, and their product specificities were altered according to the oligomerization. The MBP-fusion protein has protease-sensitive sites in the portion corresponding to geranylgeranyl diphosphate synthase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 11:47:46