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Titolo:
PLATELET-RELEASED ADP STABILIZES PAF-INDUCED RABBIT PLATELET-AGGREGATION BY STABILIZING INTRACELLULAR CALCIUM
Autore:
YI FX; GUO ZG;
Indirizzi:
HUNAN MED UNIV,MOL PHARMACOL LAB CHANGSHA 410078 PEOPLES R CHINA HUNAN MED UNIV,MOL PHARMACOL LAB CHANGSHA 410078 PEOPLES R CHINA
Titolo Testata:
Zhongguo yaoli xuebao
fascicolo: 4, volume: 19, anno: 1998,
pagine: 379 - 382
SICI:
0253-9756(1998)19:4<379:PASPRP>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEAGGREGATION; THROMBIN; MECHANISMS; ACTIVATION;
Keywords:
PLATELET AGGREGATION; PLATELET ACTIVATING FACTOR; APYRASE; ADENOSINE DIPHOSPHATE; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
F.X. Yi e Z.G. Guo, "PLATELET-RELEASED ADP STABILIZES PAF-INDUCED RABBIT PLATELET-AGGREGATION BY STABILIZING INTRACELLULAR CALCIUM", Zhongguo yaoli xuebao, 19(4), 1998, pp. 379-382

Abstract

AIM: To examine whether platelet-released adenosine diphosphate (ADP)would contribute to the stabilization of rabbit platelet aggregation induced by platelet activating factor (PAF). METHODS: Rabbit platelet aggregation induced by PAF was measured turbimetrically. ADP release from rabbit platelets stimulated by PAF was determined by HPLC. Intracellular Ca2+ was measured using Ca2+-sensitive fluorescent indicator Fura 2-AM. RESULTS: PAF greater than or equal to 1 nmol.L-1 induced fullplatelet aggregation, which did not deaggregate over 5 min after aggregation reached peak. Platelet aggregation was deaggregated in a concentration-dependent manner by subsequent addition of ADP scavenger ATP-diphosphohydrolase (apyrase) at 5 - 100 mg.L-1 PAF 3 nmol.L-1 stimulated release of.ADP (29 % vs 6 % of control), and elicited a sapid rise in intracellular calcium ([Ca2+](i)) which peaked at approximately 15 s. Then the [Ca2+](i) gradually decayed from 585 +/- 80 nmol.L-1 within 100 s to a low level (364 +/- 82 nmol.L-1). Apyrase 100 mg.L-1, added 2 min after PAF, reduced [Ca2+](i) to a lower level (171 +/- 29 nmol. L-1). CONCLUSION: Platelet-released ADP stabilizes PAF-induced rabbitplatelet aggregation by stabilizing [Ca2+](i) at elevated level.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:19:23