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Titolo:
NON-MANUAL LYSIS OF 2ND-STAGE MELOIDOGYNE JUVENILES FOR IDENTIFICATION OF PURE AND MIXED SAMPLES BASED ON THE POLYMERASE-CHAIN-REACTION
Autore:
STANTON JM; MCNICOL CD; STEELE V;
Indirizzi:
QUEENSLAND DEPT PRIMARY IND,PLANT PROTECT UNIT,MEIERS RD INDOOROOPILLY QLD 4068 AUSTRALIA
Titolo Testata:
Australasian plant pathology
fascicolo: 2, volume: 27, anno: 1998,
pagine: 112 - 115
SICI:
0815-3191(1998)27:2<112:NLO2MJ>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL-DNA;
Keywords:
ROOT-KNOT NEMATODES; DIAGNOSTICS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Stanton et al., "NON-MANUAL LYSIS OF 2ND-STAGE MELOIDOGYNE JUVENILES FOR IDENTIFICATION OF PURE AND MIXED SAMPLES BASED ON THE POLYMERASE-CHAIN-REACTION", Australasian plant pathology, 27(2), 1998, pp. 112-115

Abstract

Non-manual methods of lysing single second-stage juveniles (J2s) of Meloidogyne and direct squashing of nematodes were assessed for consistency by the success of subsequent amplification of mitochondrial DNA by polymerase chain reaction. Microwave heating and boiling resulted inamplification of DNA from only 10% of J2s; treatment with proteinase K, 20%; direct squashing, 50%; and 24 h incubation in NaOH, 81%. Components of mixtures of mtDNA types could be detected consistently by DNAamplification only if they constituted at least 30% of the mixture.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:08:37