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Titolo:
PREFERENTIAL EXPRESSION OF CHOLESTERYL ESTER TRANSFER PROTEIN MESSENGER-RNA BY STROMAL-VASCULAR CELLS OF HUMAN ADIPOSE-TISSUE
Autore:
RADEAU T; ROBB M; MCDONNELL M; MCPHERSON R;
Indirizzi:
UNIV OTTAWA,INST HEART,LIPOPROT & ATHEROSCLEROSIS GRP,LAB H453,1053 CARLING AVE OTTAWA ON K1Y 4E9 CANADA UNIV OTTAWA,INST HEART,LIPOPROT & ATHEROSCLEROSIS GRP,LAB H453,1053 CARLING AVE OTTAWA ON K1Y 4E9 CANADA
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 2-3, volume: 1392, anno: 1998,
pagine: 245 - 253
SICI:
0005-2760(1998)1392:2-3<245:PEOCET>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPID TRANSFER PROTEIN; SMALL FAT-CELLS; BINDING PROTEIN; MESSENGER-RNA; GENE-EXPRESSION; LIPOPROTEIN-LIPASE; RAT ADIPOCYTES; ACID; DIFFERENTIATION; OBESITY;
Keywords:
ADIPOSE TISSUE CELLULARITY AND METABOLISM; CHOLESTEROL; PRE-ADIPOCYTE; CHOLESTERYL ESTER TRANSFER PROTEIN; LIPOPROTEIN LIPASE; APOLIPOPROTEIN E; CCAAT ENHANCER BINDING PROTEIN ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
T. Radeau et al., "PREFERENTIAL EXPRESSION OF CHOLESTERYL ESTER TRANSFER PROTEIN MESSENGER-RNA BY STROMAL-VASCULAR CELLS OF HUMAN ADIPOSE-TISSUE", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1392(2-3), 1998, pp. 245-253

Abstract

Cholesteryl ester transfer protein (CETP) mRNA is more abundantly expressed in small mature adipocytes as compared to large, lipid-rich adipocytes [Radeau et al., J. Lipid Res. 36 (1995) 2552-2561]. In the present study, the stromal vascular fraction (SVF) of human adipose tissue was isolated and the presence of very small fat cells in this fraction confirmed by electron microscopy and by demonstrating the presence of mRNA for adipsin and for CCAAT enhancer binding protein alpha (C/EBP alpha), a marker of adipocyte differentiation. sn-Glycerol 3-phosphate dehydrogenase (GPDH) activity was present in the SVF but not in thepreadipocyte fraction. Northern blot analysis of human adipose tissuedemonstrated that CETP mRNA expression was significantly greater (+96%, P < 0.03) in stromal-vascular cells (SVC) as compared to mature fatcells. By comparison, lipoprotein lipase mRNA expression was lower (-75%, P< 0.03) in SVC while apolipoprotein E mRNA expression was not significantly different in SVC as compared to isolated adipocytes. By RT-PCR analysis, we demonstrated that CETP mRNA was expressed by human pre-adipocytes at levels less than those of SVC and adipocytes. The absence of monocytes/macrophages in SVC was confirmed by the absence of Fc gamma RIII (CD16) mRNA in these fractions. These data demonstrate that CETP mRNA is most highly expressed in the immature fat cells of human adipose tissue, consistent with other experiments from this laboratory demonstrating that CETP plays an important local role in adipocytecholesterol accumulation. (C) 1998 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 00:32:45