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Titolo:
ESTABLISHMENT AND CHARACTERIZATION OF A MEGAKARYOBLAST CELL-LINE WITHAMPLIFICATION OF MLL
Autore:
ALLEN RJ; SMITH SD; MOLDWIN RL; LU MM; GIORDANO L; VIGNON C; SUTO Y; HARDEN A; TOMEK R; VELDMAN T; RIED T; LARSON RA; LEBEAU MM; ROWLEY JD; ZELEZNIKLE N;
Indirizzi:
UNIV KANSAS,MED CTR,DELP BLDG,RM 3032,3901 RAINBOW BLVD KANSAS CITY KS 66160 UNIV CHICAGO,SECT PEDIAT HEMATOL ONCOL,DEPT PEDIAT CHICAGO IL 60637 UNIV CHICAGO,HEMATOL ONCOL SECT,DEPT MED CHICAGO IL 60637 NATL HUMAN GENOME RES INST,NIH BETHESDA MD 00000
Titolo Testata:
Leukemia
fascicolo: 7, volume: 12, anno: 1998,
pagine: 1119 - 1127
SICI:
0887-6924(1998)12:7<1119:EACOAM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE MYELOID-LEUKEMIA; CHROMOSOMAL TRANSLOCATIONS; GENE; 11Q23; REARRANGEMENTS; DIFFERENTIATION; DUPLICATION; EXPRESSION; SEQUENCES; FEATURES;
Keywords:
CELL LINE; MEGAKARYOBLAST; MLL AMPLIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
R.J. Allen et al., "ESTABLISHMENT AND CHARACTERIZATION OF A MEGAKARYOBLAST CELL-LINE WITHAMPLIFICATION OF MLL", Leukemia, 12(7), 1998, pp. 1119-1127

Abstract

A new cell line with megakaryoblastic features, designated UoC-M1, was established from the malignant cells of a 68-year-old patient with acute myeloid leukemia. The patient's leukemic cells reacted with cu-naphthyl acetate esterase and acid phosphatase and expressed CD7, CD24, CD34, CD38, CD45, HLA-DR and CD61. Cytogenetic analysis of the patient's malignant cells (and of the UoC-M1 cells) showed a human, male hypodiploid karyotype with many chromosome rearrangements and marker chromosomes. Spectral karyotyping (SKY) analysis complemented the G-banded karyotyping and clarified several chromosomal translocations and identified the marker chromosomes. Fluorescence in site hybridization (FISH) and SKY analysis demonstrated that one marker chromosome contained three segments of chromosome 9 interspersed with three segments of chromosome 11, as well as a portion of chromosome 19. FISH analysis with aprobe for MLL revealed that the UoC-M1 cells contained four copies ofthe MLL gene. Southern blot analysis determined that the MLL gene hada germline profile while Northern and Western analyses showed that the MLL mRNAs and protein were of the appropriate sizes. This is the first report of amplification of the MLL gene which may be an additional mechanism of leukemogenesis or disease progression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 06:09:50