Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DELTA-OPIOID RECEPTORS ON ASTROGLIAL CELLS IN PRIMARY CULTURE - MOBILIZATION OF INTRACELLULAR FREE CALCIUM VIA A PERTUSSIS SENSITIVE G-PROTEIN
Autore:
THORLIN T; ERIKSSON PS; PERSSON PAI; ABERG ND; HANSSON E; RONNBACK L;
Indirizzi:
GOTHENBURG UNIV,INST NEUROBIOL,MED AREGATAN 5 S-41390 GOTHENBURG SWEDEN GOTHENBURG UNIV,INST CLIN NEUROSCI,DEPT NEUROBIOL S-41390 GOTHENBURG SWEDEN
Titolo Testata:
Neuropharmacology
fascicolo: 3, volume: 37, anno: 1998,
pagine: 299 - 311
SICI:
0028-3908(1998)37:3<299:DROACI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTION-POTENTIAL DURATION; KAPPA-OPIATE RECEPTORS; ACTIVATED CA2+ INFLUX; GANGLION HYBRID-CELLS; RAT CEREBRAL-CORTEX; MESSENGER-RNA; FUNCTIONAL EXPRESSION; ADENYLATE-CYCLASE; INDUCED INCREASE; BINDING PROTEIN;
Keywords:
ASTROCYTES; CA2+ CHANNELS; DELTA-OPIOID RECEPTORS; GTP-BINDING PROTEINS; CORTEX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
74
Recensione:
Indirizzi per estratti:
Citazione:
T. Thorlin et al., "DELTA-OPIOID RECEPTORS ON ASTROGLIAL CELLS IN PRIMARY CULTURE - MOBILIZATION OF INTRACELLULAR FREE CALCIUM VIA A PERTUSSIS SENSITIVE G-PROTEIN", Neuropharmacology, 37(3), 1998, pp. 299-311

Abstract

Astrocytes in primary culture from rat cerebral cortex were probed concerning the expression of delta-opioid receptors and their coupling to changes in intracellular free calcium concentrations ([Ca2+](i)). Fluo-3 or fura-2 based microspectrofluorometry was used for [Ca2+](i) measurements on single astrocytes in a mixed astroglial-neuronal culture. Application of the selective delta-opioid receptor agonist, [D-Pen(2), D-Pen(5)]-enkephalin (DPDPE), at concentrations ranging from 10 nM to 100 mu M, induced concentration-dependent increases in [Ca2+](i) (EC50 =114 nM). The responses could be divided into two phases, with an initial spike in [Ca2+](i) followed by either oscillations or a sustained elevation of [Ca2+](i). These effects were blocked by the selective delta-opioid receptor antagonist ICI 174864 (10 mu M). The expression of delta-opioid receptors on astroglial cells was further verified immunohistochemically, using specific antibodies, and by Western blot analyses. Pre-treatment of the cells with pertussis toxin (100 ng/ml, 24 h) blocked the effects of delta-opioid receptor activation, consistent with a G(i)- or G(o)-mediated response. The sustained elevation of [Ca2+](i) was not observed in low extracellular Ca2+ and was partly blocked by nifedipine (1 mu M), indicating the involvement of L-type Ca2 channels. Stimulating neurons with DPDPE resulted in a decrease in [Ca2+](i), which may be consistent with the closure of the plasma membrane Ca2+ channels on these cells. The current results suggest a role for astrocytes in the response of the brain to delta-opioid peptides and that these opioid effects in part involve altered astrocytic intracellular Ca2+ homeostasis. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 09:46:16