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Titolo:
PEDIGREE ANALYSIS OF THE TRANSMISSION OF A DOUBLE-STRANDED-RNA IN BARLEY CULTIVARS
Autore:
ZABALGOGEAZCOA I; COXFOSTER DC; GILDOW FE;
Indirizzi:
PENN STATE UNIV,DEPT PLANT PATHOL UNIV PK PA 16802 PENN STATE UNIV,DEPT PLANT PATHOL UNIV PK PA 16802 PENN STATE UNIV,DEPT ENTOMOL UNIV PK PA 16802
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 91, anno: 1993,
pagine: 45 - 53
SICI:
0168-9452(1993)91:1<45:PAOTTO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS;
Keywords:
HORDEUM-VULGARE; DSRNA; CDNA; THERMAL REVERSE TRANSCRIPTASE; GENETIC TRANSMISSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
I. Zabalgogeazcoa et al., "PEDIGREE ANALYSIS OF THE TRANSMISSION OF A DOUBLE-STRANDED-RNA IN BARLEY CULTIVARS", PLANT SCI, 91(1), 1993, pp. 45-53

Abstract

The barley cultivar Barsoy (Hordeum vulgare L.) contains an unusual 13.1 kb cellular double-stranded ribonucleic acid (dsRNA). This non-viral dsRNA is efficiently transmitted by both egg and pollen in crosses with dsRNA-free cultivars. The pedigree of Barsoy was reconstructed and tested for dsRNA occurrence and sequence similarity in an effort to determine the origin and distribution of this molecule. The ancestors of Barsoy barley were tested for the presence of dsRNA by CF-11 cellulose chromatography and agarose electrophoresis. Of 31 ancestral cultivars tested, eight contained a dsRNA with electrophoretic properties similar to the one from Barsoy. A cDNA probe made with Barsoy dsRNA revealed that the dsRNAs isolated from related cultivars have similar nucleotide sequences. The dsRNA was found in four out of five of the oldest ancestors of Barsoy tested. One of these cultivars, Nakano Wase, wasintroduced to the US from Japan for breeding purposes in 1911. These results support a theory for vertical transmission of the dsRNA from generation to generation and suggest that the molecule has been maintained in barley cultivars for over 80 years.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:43:55