Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A MUTATION IN NPS1 STH1, AN ESSENTIAL GENE ENCODING A COMPONENT OF A NOVEL CHROMATIN-REMODELING COMPLEX RSC, ALTERS THE CHROMATIN STRUCTUREOF SACCHAROMYCES-CEREVISIAE CENTROMERES/
Autore:
TSUCHIYA E; HOSOTANI T; MIYAKAWA T;
Indirizzi:
HIROSHIMA UNIV,FAC ENGN,DEPT FERMENTAT TECHNOL HIGASHIHIROSHIMA 7398527 JAPAN
Titolo Testata:
Nucleic acids research
fascicolo: 13, volume: 26, anno: 1998,
pagine: 3286 - 3292
SICI:
0305-1048(1998)26:13<3286:AMINSA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOTIC CHROMOSOME TRANSMISSION; YEAST CENTROMERE; TRANSCRIPTIONAL ACTIVATORS; MULTISUBUNIT COMPLEX; NUCLEOSOMAL DNA; PROTEIN; PRODUCTS; HISTONE; FAMILY; SNF2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
E. Tsuchiya et al., "A MUTATION IN NPS1 STH1, AN ESSENTIAL GENE ENCODING A COMPONENT OF A NOVEL CHROMATIN-REMODELING COMPLEX RSC, ALTERS THE CHROMATIN STRUCTUREOF SACCHAROMYCES-CEREVISIAE CENTROMERES/", Nucleic acids research, 26(13), 1998, pp. 3286-3292

Abstract

The NPS1/STH1 gene encodes a nuclear protein essential for the progression of G2/M phase in Saccharomyces cerevisiae, Nps1p shares homologyto Snf2/Swi2p, a subunit of a protein complex known as the SNF/SWI complex. Recently, Nps1p was found to be a component of a protein complex termed RSC (3) essential for mitotic growth, whereas its function isunknown. We isolated a temperature-sensitive mutant allele of NPS1, nps1-105, and found that the mutation increases the sensitivity to thiabendazole (TBZ), At the restrictive temperature, nps1-105 arrested at the G2/M phase in MAD1-dependent manner and missegregated the mini-chromosome with higher frequency than the wild type cells. The nuclease digestion of the chromatin of the mutant cells revealed that the mutation causes the alteration of the chromatin structure around centromeresat the restrictive temperature, The results suggested that, in the nps1-105 mutant, impaired chromatin structure surrounding centromeres may lead to an impairment of kinetochore function and the cells arrest at G2/M phase through the spindle-assembly checkpoint system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:38:14