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Titolo:
CLONING, SEQUENCING AND LINKAGE MAPPING OF THE NRAMP1 GENE OF SHEEP AND DEER
Autore:
MATTHEWS GD; CRAWFORD AM;
Indirizzi:
UNIV OTAGO,DEPT BIOCHEM,AGRES MOL BIOL UNIT,POB 56 DUNEDIN NEW ZEALAND
Titolo Testata:
Animal genetics
fascicolo: 1, volume: 29, anno: 1998,
pagine: 1 - 6
SICI:
0268-9146(1998)29:1<1:CSALMO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-RESISTANCE; MACROPHAGE PROTEIN; INTRACELLULAR PARASITES; CDNA CLONES; ORGANIZATION; EXPRESSION; INFECTIONS; CANDIDATE; LOCUS; MAP;
Keywords:
DEER; INTRACELLULAR BACTERIA; MICROSATELLITE; NRAMP1; SHEEP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
G.D. Matthews e A.M. Crawford, "CLONING, SEQUENCING AND LINKAGE MAPPING OF THE NRAMP1 GENE OF SHEEP AND DEER", Animal genetics, 29(1), 1998, pp. 1-6

Abstract

The ovine NRAMP1 and cervine NRAMP1 cDNAs were cloned by RT PCR of RNA derived from macrophage enriched leukocyte preparations. The complete coding and 3' regions were sequenced. Both sheep and deer NRAMP1 proteins are 548 amino acids long. There are 77 and 73 amino acid differences, respectively, compared to the mouse Nramp1 sequence. Dinucleotide repeats were found in both the ovine and cervine 3' non-coding sequence. Amplification of these regions in individual sheep and deer showed them to be polymorphic microsatellites. They have polymorphism information content values of 0.76 and 0.84 in sheep and deer, respectively. Using these microsatellites, the ovine NRAMP1 gene was mapped in a linkage group on ovine chromosome 2q and cervine NRAMP1 was mapped in alinkage group syntenic with human chromosome 2, mouse chromosome 1 and sheep chromosome 2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:31:41