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Titolo:
A RELATIONSHIP BETWEEN PROTEINASE ACTIVITY AND CLINICAL-PARAMETERS INTHE TREATMENT OF PERIODONTAL-DISEASE
Autore:
MAILHOT JM; POTEMPA J; STEIN SH; TRAVIS J; STERRETT JD; HANES PJ; RUSSELL CM;
Indirizzi:
MED COLL GEORGIA,SCH DENT,DEPT PERIODONTOL,1459 LANEY WALKER BLVD AUGUSTA GA 30912 MED COLL GEORGIA,DEPT ORAL BIOL AUGUSTA GA 30912 MED COLL GEORGIA,DEPT BIOSTAT AUGUSTA GA 30912 UNIV GEORGIA,DEPT BIOCHEM ATHENS GA 30602 JAGIELLONIAN UNIV,INST MOL BIOL KRAKOW POLAND
Titolo Testata:
Journal of clinical periodontology
fascicolo: 7, volume: 25, anno: 1998,
pagine: 578 - 584
SICI:
0303-6979(1998)25:7<578:ARBPAA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GINGIVAL CREVICULAR FLUID; TRYPSIN-LIKE ACTIVITY; PORPHYROMONAS-GINGIVALIS; ATTACHMENT LOSS; GRANULOCYTE ELASTASE; CYSTEINE PROTEINASE; SUBGINGIVAL PLAQUE; HOST RESPONSE; PATHOGENESIS; PROGRESSION;
Keywords:
PORPHYROMONAS-GINGIVALIS; PROTEINASE; ELASTASE; CATHEPSIN G; GINGIVAL CREVICULAR FLUID; PERIODONTAL DISEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Mailhot et al., "A RELATIONSHIP BETWEEN PROTEINASE ACTIVITY AND CLINICAL-PARAMETERS INTHE TREATMENT OF PERIODONTAL-DISEASE", Journal of clinical periodontology, 25(7), 1998, pp. 578-584

Abstract

The objective of this research was to determine the effectiveness of a biochemical assay which measures proteolytic enzyme activity in gingival crevicular fluid (GCF) and to relate this enzyme activity to clinical parameters traditionally utilized for periodontitis detection. A clinical trial was conducted on 8 periodontitis subjects with greater than or equal to 4 sites exhibiting a loss of attachment of greater than or equal to 5 mm and probing depths of greater than or equal to 5 mm with bleeding on probing. On each subject; a plaque index was performed, followed by GCF sampling at those sites which exhibited a loss ofattachment and probing depths. GCF was analyzed for activity against benzoyl-L-arginine-p-nitroanilide in the presence (BAPNA w/gly-gly) and the absence (BAPNA w/o gly-gly) of glycyl-glycine and against MeOSuc-Ala-Ala-Pro-Val-pNA and Suc-Ala-Ala-Pro-Phe-pNA for neutrophil serineproteinases activity (elastase and cathepsin G, respectively). Subsequently, a gingival index was performed, attachment levels and probing depths were recorded using a constant force probe with bleeding on probing being noted. A split-mouth design was employed and half mouths were randomly assigned to the following treatment groups: group A, half of the mouth received scaling/root planing and polishing: group B, half of the mouth received no treatment (control). Subjects were treated,then instructed on toothbrushing and interdental cleaning. After 4 weeks, subjects returned to receive a plaque index, GCF sampling, gingival index, attachment levels, probing depths and bleeding on probing asdescribed above. Using a paired Student t-test, the findings suggest that BAPNA w/gly-gly was significantly less in treatment sites than innon-treated control sites (p=0.05). No such correlation was found forother activities, including neutrophil serine proteinases which were shown to occur in GCF in free, proteolytically active forms. In addition, significant treatment effects were detected fur probing depths (p=0.03) which reduced by 1.3 mm and attachment levels (p=0.02) which gained 0.7 mm. The reduction of P. gingivalis From treated periodontitis sites as detected by a significant decrease in BAPNA w/gly-gly may prove to be a valuable marker for periodontal disease activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 16:14:56