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Titolo:
GABAERGIC INPUT TO CHOLINERGIC NUCLEUS BASALIS NEURONS
Autore:
KHATEB A; FORT P; WILLIAMS S; SERAFIN M; MUHLETHALER M; JONES BE;
Indirizzi:
MCGILL UNIV,DEPT NEUROL & NEUROSURG,MONTREAL NEUROL INST,3801 UNIV STMONTREAL PQ H3A 2B4 CANADA MCGILL UNIV,DEPT NEUROL & NEUROSURG,MONTREAL NEUROL INST MONTREAL PQ H3A 2B4 CANADA CMU,DEPT PHYSIOL CH-1211 GENEVA 4 SWITZERLAND
Titolo Testata:
Neuroscience
fascicolo: 3, volume: 86, anno: 1998,
pagine: 937 - 947
SICI:
0306-4522(1998)86:3<937:GITCNB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIPPOCAMPAL PYRAMIDAL CELLS; RECEPTOR-MEDIATED RESPONSES; GYRUS GRANULE CELLS; GUINEA-PIG BRAIN; FOREBRAIN NEURONS; RAT HIPPOCAMPUS; IMMUNOHISTOCHEMICAL EVIDENCE; ACETYLCHOLINE-RELEASE; SYNAPTIC RESPONSES; GABA(A) RECEPTORS;
Keywords:
BASAL FOREBRAIN; GLUTAMATE DECARBOXYLASE; CHOLINE ACETYLTRANSFERASE; GABA(A) RECEPTORS; MUSCIMOL; SLEEP-WAKE STATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
A. Khateb et al., "GABAERGIC INPUT TO CHOLINERGIC NUCLEUS BASALIS NEURONS", Neuroscience, 86(3), 1998, pp. 937-947

Abstract

The potential influence of GABAergic input to cholinergic basalis neurons was studied in guinea-pig basal forebrain slices. GABA and its agonists were applied to electrophysiologically-identified cholinergic neurons, of which some were labelled with biocytin and confirmed to be choline acetyltransferase-immunoreactive. Immunohistochemistry for glutamate decarboxylase was also performed in some slices and revealed GABAergic varicosities in the vicinity of the biocytin-filled soma and dendrites of electophysiologically-identified cholinergic cells. From rest (average - 63 mV), the cholinergic cells were depolarized by GABA. The depolarization was associated with a decrease in membrane resistance and diminution in firing. The effect was mimicked by muscimol, thespecific agonist for GABA(A) receptors, and not by baclofen; the specific agonist For GABA(B) receptors, which had no discernible effect. The GABA- and muscimol-evoked depolarization and decrease in resistancewere found to be postsynaptic since they persisted in the presence ofsolutions containing either high Mg2+/low Ca2+ or tetrodotoxin. They were confirmed as being mediated by a GABA(A) receptor, since they were antagonized by bicuculline. The reversal potential for the muscimol effect was estimated to be similar to - 45 mV, which was -15 mV above the resting membrane potential. Finally, in some cholinergic cells, spontaneous subthreshold depolarizing synaptic potentials (average 5 mV in amplitude), which were rarely associated with action potentials, were recorded and found to persist in the presence of glutamate receptorantagonists but to be eliminated by bicuculline. These results suggest that GABAergic input map be depolarizing, yet predominantly inhibitory to cholinergic basalis neurons. (C) 1998 IBRO. Published by Elsevier Science Ltd.

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Documento generato il 07/07/20 alle ore 12:55:52