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Titolo:
DISRUPTION OF THE RETINAL BASAL LAMINA DURING EARLY EMBRYONIC-DEVELOPMENT LEADS TO A RETRACTION OF VITREAL END FEET, AN INCREASED NUMBER OFGANGLION-CELLS, AND ABERRANT AXONAL OUTGROWTH
Autore:
HALFTER W;
Indirizzi:
UNIV PITTSBURGH,DEPT NEUROBIOL,1414 W BIOL SCI TOWER PITTSBURGH PA 15261
Titolo Testata:
Journal of comparative neurology
fascicolo: 1, volume: 397, anno: 1998,
pagine: 89 - 104
SICI:
0021-9967(1998)397:1<89:DOTRBL>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPARAN-SULFATE PROTEOGLYCAN; BASEMENT-MEMBRANE PROTEINS; EXTRACELLULAR-MATRIX; L1-LIKE MOLECULE; CORNEAL STROMA; CHICKEN RETINA; NEURAL RETINA; GROWTH; COLLAGEN; GUIDANCE;
Keywords:
COLLAGENASE; RETINA; AXONAL GUIDANCE; NEUROEPITHELIAL CELLS; EXTRACELLULAR MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
W. Halfter, "DISRUPTION OF THE RETINAL BASAL LAMINA DURING EARLY EMBRYONIC-DEVELOPMENT LEADS TO A RETRACTION OF VITREAL END FEET, AN INCREASED NUMBER OFGANGLION-CELLS, AND ABERRANT AXONAL OUTGROWTH", Journal of comparative neurology, 397(1), 1998, pp. 89-104

Abstract

Bacterial collagenase was injected into the vitreous of the eye of chick and quail embryos. Immunocytochemical and ultrastructural studies revealed that the collagenase dissolved the retinal basal lamina of the injected eye. The basal lamina disruption was first detectable 1 hour after enzyme injection and was complete within 3 hours. With furtherdevelopment, the retinal basal lamina was not reestablished; newly developing neuroepithelium in the peripheral retina, however, generated an intact basal lamina. Western blot analysis showed that Clostridial collagenase degraded various collagens but spared noncollagenous proteins. Basal lamina disruption of embryonic day 3 to 6 retinae led to the retraction of the end feet of the neuroepithelial cells, caused an increase in the number of Islet-1(+) cells (most likely ganglion cells), an increase in the thickness of the optic fiber layer, and aberrant growth of optic axons on their way toward the optic disc. None of these changes were observed when retinal basal laminae were disrupted at later stages of development. The present data demonstrate that the retinal basal lamina, by anchoring the neuroepithelial cells to the pial surface of the retina, has an important function in the development of the normal cytoarchitecture of this structure. It is proposed that thealtered extracellular environment in the vitreal part of the retina, resulting in the retraction of the neuroepithelial end feet, is responsible for the increased number of Islet-1(+) cells and the aberrant axonal navigation. (C) 1998 Wiley-Liss, Inc.

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Documento generato il 31/03/20 alle ore 21:36:31