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Titolo:
MIRANDA MEDIATES ASYMMETRIC PROTEIN AND RNA LOCALIZATION IN THE DEVELOPING NERVOUS-SYSTEM
Autore:
SCHULDT AJ; ADAMS JHJ; DAVIDSON CM; MICKLEM DR; HASELOFF J; STJOHNSTON D; BRAND AH;
Indirizzi:
WELLCOME CRC INST CAMBRIDGE CB2 1QR ENGLAND WELLCOME CRC INST CAMBRIDGE CB2 1QR ENGLAND UNIV CAMBRIDGE,DEPT GENET CAMBRIDGE CB2 1QR ENGLAND MRC,MOL BIOL LAB CAMBRIDGE CB2 2QH ENGLAND
Titolo Testata:
Genes & development
fascicolo: 12, volume: 12, anno: 1998,
pagine: 1847 - 1857
SICI:
0890-9369(1998)12:12<1847:MMAPAR>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; OSKAR MESSENGER-RNA; DROSOPHILA OOCYTE; BINDING DOMAIN; PROSPERO GENE; MOTHER CELLS; NUMB; MUTATIONS; STAUFEN; FATES;
Keywords:
RNA LOCALIZATION; ASYMMETRIC SEGREGATION; MIRANDA; STAUFEN; PROSPERO; NERVOUS SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Schuldt et al., "MIRANDA MEDIATES ASYMMETRIC PROTEIN AND RNA LOCALIZATION IN THE DEVELOPING NERVOUS-SYSTEM", Genes & development, 12(12), 1998, pp. 1847-1857

Abstract

Neuroblasts undergo asymmetric stem cell divisions to generate a series of ganglion mother cells (GMCs). During these divisions, the cell fate determinant Prospero is asymmetrically partitioned to the GMC by Miranda protein, which tethers it to the basal cortex of the dividing neuroblast. Interestingly, prospero mRNA is similarly segregated by thedsRNA binding protein, Staufen. Here we show that Staufen interacts in vivo with a segment of the prospero 3' UTR. Staufen protein and prospero RNA colocalize to the apical side of the neuroblast at interphase, but move to the basal side during prophase. Both the apical and basal localization of Staufen are abolished by the removal of a conserved domain from the carboxyl terminus of the protein, which interacts in ayeast two-hybrid screen with Miranda protein. Furthermore, Miranda colocalizes with Staufen protein and prospero mRNA during neuroblast divisions, and neither Staufen nor prospero RNA are localized in miranda mutants. Thus Miranda, which localizes Prospero protein, also localizes prospero RNA through its interaction with Staufen protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:58:24