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Titolo:
CLONING OF THE ASPERGILLUS-AWAMORI GLUCOAMYLASE GENE AND EXPRESSION IN SACCHAROMYCES-CEREVISIAE
Autore:
WANG YJ; LIU HD; SUN T; ZHANG SH;
Indirizzi:
CHINESE ACAD SCI,INST MICROBIOL BEIJING 100080 PEOPLES R CHINA CHINESE ACAD SCI,INST MICROBIOL BEIJING 100080 PEOPLES R CHINA
Titolo Testata:
Chinese Science Bulletin
fascicolo: 10, volume: 43, anno: 1998,
pagine: 855 - 859
SICI:
1001-6538(1998)43:10<855:COTAGG>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
YEAST; DNA;
Keywords:
ASPERGILLUS AWAMORI; GLUCOAMYLASE; GENE CLONING AND EXPRESSION; SACCHAROMYCES CEREVISIAE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
Y.J. Wang et al., "CLONING OF THE ASPERGILLUS-AWAMORI GLUCOAMYLASE GENE AND EXPRESSION IN SACCHAROMYCES-CEREVISIAE", Chinese Science Bulletin, 43(10), 1998, pp. 855-859

Abstract

The A. awamori glucoamylase I gene was obtained by RT-PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1-GA, which was used to to transform the yeast S. cerevisiae AS 2.1364 without auxotrophic marker. The transformant secreted glucoamylase into themedium efficiently and degraded starch. The glucoamylase activity of the culture filtrate is up to 8.4 U/mL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 18:24:48