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Titolo:
HUMAN CHROMOSOME-11 COMPLEMENTS ATAXIA-TELANGIECTASIA CELLS BUT DOES NOT COMPLEMENT THE DEFECT IN AT-LIKE CHINESE-HAMSTER CELL MUTANTS
Autore:
JONGMANS W; WIEGANT J; OSHIMURA M; JAMES MR; LOHMAN PHM; ZDZIENICKA MZ;
Indirizzi:
LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,MGC,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,MGC,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS INTERUNIV RES INST RADIOPATHOL & RADIAT PROTECT,JA COHEN INST LEIDEN NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY,MGC 2333 AL LEIDEN NETHERLANDS TOTTORI UNIV,DEPT MOLEC & CELL GENET YONAGO TOTTORI 683 JAPAN HUMAN POLYMORPHISM STUDY CTR F-75010 PARIS FRANCE
Titolo Testata:
Human genetics
fascicolo: 3, volume: 92, anno: 1993,
pagine: 259 - 264
SICI:
0340-6717(1993)92:3<259:HCCACB>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
NIJMEGEN BREAKAGE SYNDROME; DNA-REPAIR GENE; IONIZING-RADIATION; INSTABILITY DISORDER; RESISTANCE; LOCUS; IMMUNODEFICIENCY; LOCALIZATION; SENSITIVITY; PHENOTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
W. Jongmans et al., "HUMAN CHROMOSOME-11 COMPLEMENTS ATAXIA-TELANGIECTASIA CELLS BUT DOES NOT COMPLEMENT THE DEFECT IN AT-LIKE CHINESE-HAMSTER CELL MUTANTS", Human genetics, 92(3), 1993, pp. 259-264

Abstract

It has been shown that the X-ray-sensitive Chinese hamster V79 mutants (V-E5, V-C4 and V-G8) are similar to ataxia-telangiectasia (A-T) cells. To determine whether the AT-like rodent cell mutants are defectivein the gene homologous to A-T (group A, C or D), human chromosome 11 was introduced to the V-E5 and V-G8 mutant cells by microcell-mediatedchromosome transfer. Forty independent hybrid clones were obtained inwhich the presence of chromosome 11 was determined by in situ hybridization. The presence of the region of chromosome 11q22-23 was shown bymolecular analysis using polymorphic DNA markers specific for the ATA, ATC and ATD loci. Seventeen of the obtained monochromosomal Chinese hamster hybrids contained a cytogenetically normal human chromosome 11, but only twelve hybrid cell lines were shown to contain an intact 11q22-23 region. Despite the complementation of the X-ray sensitivity bya normal chromosome 11 introduced to A-T cells (complementation groupD), these twelve Chinese hamster hybrid clones showed lack of complementation of X-ray and streptonigrin hypersensitivity. The observed lack of complementation does not seem to be attributable to hypermethylation of the human chromosome 11 in the rodent cell background, since 5-azacytidine treatment had no effect on the streptonigrin hypersensitivity of the hybrid cell lines. These results indicate that the gene defective in the AT-like rodent cell mutants is not homologous to the ATA, ATC or ATD genes and that the human gene complementing the defect inthe AT-like mutants seems not to be located on human chromosome 11.

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Documento generato il 28/11/20 alle ore 15:48:31