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Titolo:
IMPACT OF CHRONIC TREATMENT OF EWES WITH ESTRADIOL-17-BETA OR PROGESTERONE ON OXYTOCIN RECEPTOR GENE-TRANSCRIPTION AND OVARIAN OXYTOCIN SECRETION
Autore:
HAZZARD TM; PINCKARD KL; STORMSHAK F;
Indirizzi:
OREGON STATE UNIV,DEPT ANIM SCI CORVALLIS OR 97331 OREGON STATE UNIV,DEPT ANIM SCI CORVALLIS OR 97331 OREGON STATE UNIV,DEPT BIOCHEM & BIOPHYS CORVALLIS OR 97331
Titolo Testata:
Biology of reproduction
fascicolo: 1, volume: 59, anno: 1998,
pagine: 105 - 110
SICI:
0006-3363(1998)59:1<105:IOCTOE>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESTROUS-CYCLE; CORPUS-LUTEUM; OVINE ENDOMETRIUM; EARLY-PREGNANCY; IN-VIVO; UTERINE; PROSTAGLANDIN-F2-ALPHA; RELEASE; INVITRO; SHEEP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
T.M. Hazzard et al., "IMPACT OF CHRONIC TREATMENT OF EWES WITH ESTRADIOL-17-BETA OR PROGESTERONE ON OXYTOCIN RECEPTOR GENE-TRANSCRIPTION AND OVARIAN OXYTOCIN SECRETION", Biology of reproduction, 59(1), 1998, pp. 105-110

Abstract

Three experiments were conducted, the objectives of which were to 1) examine the effects of exogenous estradiol (E-2) and progesterone (P-4) on uterine concentrations of oxytocin receptors (OTR) and OTR mRNA, as well as the effect of exogenous P-4 on progesterone receptors (PR) during the late luteal phase of the cycle, and 2) ascertain whether chronic E-2 treatment of ewes during the cycle would alter prostaglandinF-2 alpha (PGF(2 alpha))-induced secretion of luteal oxytocin (OT). In experiment 1, 15 ewes were assigned to a control (n = 5; 2 ml corn oil [CO] s,c, on Days 4-14 of the estrous cycle) and two treatment groups in = 5 each) receiving either 250 mu 8 E-2 s,c. (Days 4-14) or 10 mg P-4 s.c. (CO on Days 4-10 and P-4 on Days 11-14). Endometria and corpora lutea were removed on Day 15 of the cycle, Mean luteal weights were greater in treated than in control ewes (p < 0.05). Endometrial concentrations of OTR and OTR mRNA were significantly greater in control than in E-2- or P-4-treated ewes, In experiment 2, five ewes each weretreated s.c. with CO or 10 mg P-4 on Days 11-14 of the cycle; endometria were then removed on Day 15 for PR assay. Endometrial concentration of PR did not differ between groups. Experiment 3 consisted of 20 ewes assigned to four groups in a 2 x 2 factorial arrangement. Treatmentconsisted of two dosages of E-2 (0 or 250 mu g/day) in 2 mi CO and two dosages of PGF(2 alpha) analogue (0 or 125 mu g Estrumate). All eweswere injected s.c. with E-2 or CO for 11 days as described for experiment 1. On Day 15, all ewes received an i.v. injection of PCF2 alpha or saline (Time 0); then jugular blood was collected at frequent intervals for analysis of serum concentrations of OT. PCF2 alpha induced a release of OT in control and E(2-)treated ewes (p < 0.05) compared to the value in saline-treated ewes. Collectively, these data suggest thatin cycling ewes, exposure of the uterus to increased concentrations of E-2- or P-4 causes down-regulation of OTR as a consequence of suppression of the OTR gene. Chronic E-2 treatment of ewes during the cycle does not act directly on the ovary to alter the stores of luteal OT.

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Documento generato il 20/09/20 alle ore 07:46:55