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Titolo:
SERIAL BONE-MARROW SAMPLING FOR LONG-TERM FOLLOW-UP OF HUMAN HEMATOPOIESIS IN NOD SCID MICE/
Autore:
VERLINDEN SFF; VANES HHG; VANBEKKUM DW;
Indirizzi:
INTROGENE BV,POB 2084 NL-2301 CA LEIDEN NETHERLANDS LEIDEN UNIV,FAC MED,DEPT BIOL MOL,GENE THERAPY SECT NL-2300 RA LEIDENNETHERLANDS
Titolo Testata:
Experimental hematology
fascicolo: 7, volume: 26, anno: 1998,
pagine: 627 - 630
SICI:
0301-472X(1998)26:7<627:SBSFLF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELLS;
Keywords:
NOD/SCID MICE; XENOTRANSPLANTATION; HUMAN UMBILICAL CORD BLOOD; BONE MARROW PUNCTURE; MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
4
Recensione:
Indirizzi per estratti:
Citazione:
S.F.F. Verlinden et al., "SERIAL BONE-MARROW SAMPLING FOR LONG-TERM FOLLOW-UP OF HUMAN HEMATOPOIESIS IN NOD SCID MICE/", Experimental hematology, 26(7), 1998, pp. 627-630

Abstract

The study of long-term human hematopoiesis in immunodeficient mice isgreatly facilitated by sequential bone marrow (BM) sampling in individual animals. Until now, however, the only way to obtain these sampleswas by sacrificing the mice. In this paper we describe a novel technique for obtaining BM cells by aspiration from the femur of living mice. The technique is simple and efficient and does not disable the animals. On average 1.6 +/- 1 x 10(6) nucleated cells can be collected fromone femur at a time, which is sufficient for flow cytometry analysis,cytospin preparations, and polymerase chain reaction assays. The cellular composition of the samples obtained by puncture is identical to that of BM harvested by flushing the femur after sacrificing the animals. We present the results of 81 punctures of the femur in NOD/SCID chimeras engrafted with Ficoll-separated or CD34(bright) purified cells from human umbilical cord blood.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:40:13