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Titolo:
ASSESSMENT OF PROLIFERATIVE AND COLONY-FORMING CAPACITY AFTER SUCCESSIVE IN-VITRO DIVISIONS OF SINGLE HUMAN CD34(+) CELLS INITIALLY ISOLATED IN G(0)
Autore:
GOTHOT A; PYATT R; MCMAHEL J; RICE S; SROUR EF;
Indirizzi:
INDIANA UNIV,SCH MED,DIV HEMATOL ONCOL,1044 W WALNUT ST,R4-202 INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,DIV HEMATOL ONCOL INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,INDIANA ELKS CANC RES CTR,DEPT MED INDIANAPOLIS IN 46202 INDIANA UNIV,SCH MED,HERMAN B WELLS CTR PEDIAT RES,DEPT PEDIAT INDIANAPOLIS IN 46202
Titolo Testata:
Experimental hematology
fascicolo: 7, volume: 26, anno: 1998,
pagine: 562 - 570
SICI:
0301-472X(1998)26:7<562:AOPACC>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; EX-VIVO EXPANSION; MURINE MARROW-CELLS; HUMAN BONE-MARROW; C-KIT LIGAND; STEM-CELLS; IN-VITRO; FLT3 LIGAND; CORD-BLOOD; GROWTH-FACTORS;
Keywords:
HUMAN; CD34; HEMATOPOIESIS; CELL CYCLE; HOECHST 33342; PYRONIN Y;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Gothot et al., "ASSESSMENT OF PROLIFERATIVE AND COLONY-FORMING CAPACITY AFTER SUCCESSIVE IN-VITRO DIVISIONS OF SINGLE HUMAN CD34(+) CELLS INITIALLY ISOLATED IN G(0)", Experimental hematology, 26(7), 1998, pp. 562-570

Abstract

Exit of primitive hematopoietic progenitor cells (HPCs) from the G(0)phase of the cell cycle in response to in vitro cytokine stimulation is a limiting step in successful ex vivo expansion. Simultaneous DNA/RNA staining with Hoechst 33342 and pyronin Y was used to separate human bone marrow CD34(+) cells residing in G(0) (G(0)CD34(+)) from those cycling in G(1) and S/G(2)+M. Compared with CD34(+) cells isolated in G(1), G(0)CD34(+) cells were characterized by a delayed response to cytokine stimulation and were enriched for long-term hematopoietic culture-initiating cells. We next compared the activation kinetics of individually sorted G(0)CD34(+) cells stimulated with stem cell factor (SCF), flt3-ligand (FL), or interleukin-3 (IL-3) as single factors. In a novel clonal proliferation assay, the functional status of cells that had remained quiescent after an initial 7-day period and of those that had completed successive division cycles under each of these three factors was evaluated by assessment of subsequent proliferative capacity and maintenance of colony-forming cell precursor (pre-CFC) activity. All three cytokines were equally able to support the survival of primitive HPCs in the absence of cell division. Cells that did not respond to any cytokine stimulation for 7 days retained higher proliferative and pre-CFC activities than dividing cells. The hematopoietic function of cells that divided in response to SCF, FL, or IL-3 decreased after each division cycle. However, G(0)CD34(+) cells displayed a heterogeneous response pattern to cytokine stimulation whereby SCF appeared to have a superior ability to promote the cycling of cells with high proliferative and pre-CFC activities. These results indicate that HPCs reside in opposing hierarchies of hematopoietic potential and responsiveness to cytokine stimulation. The data also begin to indicate relationships between cellular division in response to different stimuli and maintenance of hematopoietic function.

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Documento generato il 06/07/20 alle ore 05:04:59