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Titolo:
ACTIVATION REGIMENS TO PREPARE BOVINE OOCYTES FOR INTRACYTOPLASMIC SPERM INJECTION
Autore:
RHO GJ; WU B; KAWARSKY S; LEIBO SP; BETTERIDGE KJ;
Indirizzi:
UNIV GUELPH,ONTARIO VET COLL,DEPT BIOMED SCI GUELPH ON N1G 2W1 CANADA UNIV GUELPH,ONTARIO VET COLL,DEPT BIOMED SCI GUELPH ON N1G 2W1 CANADA
Titolo Testata:
Molecular reproduction and development
fascicolo: 4, volume: 50, anno: 1998,
pagine: 485 - 492
SICI:
1040-452X(1998)50:4<485:ARTPBO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR TRANSFER EMBRYOS; PARTHENOGENETIC DEVELOPMENT; MOUSE OOCYTES; PROTEIN-KINASES; INVITRO; FERTILIZATION; SPERMATOZOA; ETHANOL; RABBIT; CATTLE;
Keywords:
IONOMYCIN; 6-DIMETHYLAMINOPURINE; ACTIVATION; PARTHENOTE; INTRACYTOPLASMIC SPERM INJECTION; BOVINE OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
G.J. Rho et al., "ACTIVATION REGIMENS TO PREPARE BOVINE OOCYTES FOR INTRACYTOPLASMIC SPERM INJECTION", Molecular reproduction and development, 50(4), 1998, pp. 485-492

Abstract

Activation of bovine oocytes to produce a single haploid pronucleus in preparation for intracytoplasmic sperm injection (ICSI) has been investigated with Various combinations of ionomycin and 6-dimethylaminopurine (DMAP). Effects were evaluated by immunocytochemical staining, chromosomal analysis and assessment of development in vitro. Oocytes matured in vitro were exposed to: ionomycin alone (single or repeated treatments, Groups 1 and 2 respectively), ionomycin followed by DMAP (immediately or after a 3-h delay, Groups 3 and 4), or no treatment (control, Group 5). They were then co-cultured in M199 with bovine oviductalepithelial cells. Activation rates were not significantly different among groups but significantly fewer oocytes in Group 3 extruded a second polar body than in Groups 1, 2, and 4. Most parthenotes (60% to 80%) in Groups 1, 2, and 4 were haploid, whereas 82% in Group 3 were mixoploid or polyploid. Most of the parthenotes (88%) in Group 4 formed a single pronucleus besides extruding the second polar body and were therefore more suitable for ICSI than those of Groups 1 and 2 in which condensed chromosomes predominated. The respective rates of oocyte cleavage in Groups 1 to 4 were 24%, 36%, 70%, and 75%; corresponding blastocyst rates were 1%, 5%, 17%, and 8%. There were significantly fewer cells in the parthenotes of Groups 1, 2, and 4 than of Group 3, or of embryos produced by in vitro fertilization. Thus, delaying the addition of DMAP after ionomycin decreases chromosomal abnormalities and produces a high proportion of activated oocytes suitable for ICSI. Mol. Reprod. Dev. 50:485-492, 1998. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 02:53:37