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Titolo:
PRODUCTION OF INTERLEUKIN-10 BY GRANULOCYTE-COLONY-STIMULATING FACTOR-MOBILIZED BLOOD PRODUCTS - A MECHANISM FOR MONOCYTE-MEDIATED SUPPRESSION OF T-CELL PROLIFERATION
Autore:
MIELCAREK M; GRAF L; JOHNSON G; TOROKSTORB B;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV CLIN RES,TRANSPLANTAT BIOL PROGRAM,1124 COLUMBIA ST,M-318 SEATTLE WA 98104
Titolo Testata:
Blood
fascicolo: 1, volume: 92, anno: 1998,
pagine: 215 - 222
SICI:
0006-4971(1998)92:1<215:POIBGF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOKINE PRODUCTION; STEM-CELLS; IL-10; MARROW; TRANSPLANTATION; SUBSETS; CLONES; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Mielcarek et al., "PRODUCTION OF INTERLEUKIN-10 BY GRANULOCYTE-COLONY-STIMULATING FACTOR-MOBILIZED BLOOD PRODUCTS - A MECHANISM FOR MONOCYTE-MEDIATED SUPPRESSION OF T-CELL PROLIFERATION", Blood, 92(1), 1998, pp. 215-222

Abstract

Previous reports showed that granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMC) are hyporesponsive to alloantigen compared with control PBMC. In the current study, neutralizing antibodies to interleukin-10 (IL-10) increased the proliferative response of G-PBMC to alloantigen by 50.14% (+/- 12.79%; n= 8), whereas the proliferative response of control PBMC was not affected. The inhibition of OKT3-stimulated CD4 cell proliferation by G-PBMC-derived CD14(+) cells could also be abrogated by the addition of IL-10 neutralizing antibodies. Further, IL-10 levels correlated with thenumber of CD14 cells in these cultures. Constitutive IL-10 mRNA levels detected by quantitative reverse transcriptase polymerase chain reaction (RT-PCR) were 10-fold higher in G-PBMC compared with control PBMC, This translated into significantly higher IL-10 levels after 24-hourlipopolysaccharide (LPS) stimulation of G-PBMC compared with control PBMC (P = .036), IL-10 mRNA levels were also fivefold higher in isolated G-PBMC-derived CD14 cells compared with control CD14 cells. This corresponded to increased constitutive production of IL-10 by isolated G-PBMC-derived CD14 cells compared with control CD14 cells (357.2 +/- 104.5 v 51.7 +/- 30.5, P = .051). In conclusion, these data suggest that monocytes contained within G-PBMC, which, in comparison to marrow, are increased in absolute number and relative proportion to T cells, may suppress T-cell responsiveness by secretion of IL-10. (C) 1998 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:49:10