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Titolo:
IMPROVING IMAGE QUALITY BY ZERO-LOSS ENERGY-FILTERING - QUANTITATIVE ASSESSMENT BY MEANS OF IMAGE CROSS-CORRELATION
Autore:
GRIMM R; TYPKE D; BAUMEISTER W;
Indirizzi:
MAX PLANCK INST BIOCHEM,ABT MOL STRUKTURBIOL D-82152 MARTINSRIED GERMANY MAX PLANCK INST BIOCHEM,ABT MOL STRUKTURBIOL D-82152 MARTINSRIED GERMANY
Titolo Testata:
Journal of Microscopy
, volume: 190, anno: 1998,
parte:, 3
pagine: 339 - 349
SICI:
0022-2720(1998)190:<339:IIQBZE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-MICROSCOPY; TEM; CONTRAST; RATIO; MODE; ICE;
Keywords:
CONSTANT ENHANCEMENT; ZERO-LOSS ENERGY FILTERING; FOURIER RING CORRELATION; ROOT-MEAN-SQUARE CONTRAST;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
R. Grimm et al., "IMPROVING IMAGE QUALITY BY ZERO-LOSS ENERGY-FILTERING - QUANTITATIVE ASSESSMENT BY MEANS OF IMAGE CROSS-CORRELATION", Journal of Microscopy, 190, 1998, pp. 339-349

Abstract

Fourier ring correlation and root-mean-square contrast of pairs of images, taken under identical conditions, were used as criteria of imagequality for comparing unfiltered with zero-loss energy-filtered imaging using a TEM equipped with a post-column energy filter. For three different specimens (amorphous carbon film, macromolecules in light negative stain, virus particles in deep negative stain) the dependence of these quantities on electron dose, specimen thickness and defocus was investigated. A model, based on simple assumptions, was used to describe quantitatively their dependence on electron dose and specimen thickness. It was found that energy filtering is most advantageous for low-dose imaging and small defocus values. The gain due to energy filtering strongly increases with specimen thickness, whereby the dependence is linear for light scattering elements. For thick specimens, the gain by energy filtering is more pronounced in the resolution range between4 and 2 nm than for lower spatial frequencies.

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Documento generato il 07/08/20 alle ore 07:29:56