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Titolo:
THIAMIN BIOSYNTHESIS IN ESCHERICHIA-COLI - IDENTIFICATION OF THIS THIOCARBOXYLATE AS THE IMMEDIATE SULFUR DONOR IN THE THIAZOLE FORMATION
Autore:
TAYLOR SV; KELLEHER NL; KINSLAND C; CHIU HJ; COSTELLO CK; BACKSTROM AD; MCLAFFERTY FW; BEGLEY TP;
Indirizzi:
CORNELL UNIV,BAKER LAB,DEPT CHEM ITHACA NY 14853 CORNELL UNIV,BAKER LAB,DEPT CHEM ITHACA NY 14853
Titolo Testata:
The Journal of biological chemistry
fascicolo: 26, volume: 273, anno: 1998,
pagine: 16555 - 16560
SICI:
0021-9258(1998)273:26<16555:TBIE-I>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MASS-SPECTROMETRY; BIOTIN SYNTHASE; SALMONELLA-TYPHIMURIUM; PYRIMIDINE MOIETY; CONVERTING FACTOR; GENE-PRODUCT; LIPOIC ACID; ORIGIN; ATOM; MOLYBDOPTERIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S.V. Taylor et al., "THIAMIN BIOSYNTHESIS IN ESCHERICHIA-COLI - IDENTIFICATION OF THIS THIOCARBOXYLATE AS THE IMMEDIATE SULFUR DONOR IN THE THIAZOLE FORMATION", The Journal of biological chemistry, 273(26), 1998, pp. 16555-16560

Abstract

ThiFSGH and ThiI are required for the biosynthesis of the thiazole moiety of thiamin in Escherichia coli. The overproduction, purification,and characterization of ThiFS and the identification of two of the early steps in the biosynthesis of the thiazole moiety of thiamin are described here. ThiS isolated from E. coli thiI(+) is post-translationally modified by converting the carboxylic acid group of the carboxyl-terminal glycine into a thiocarboxylate. The thiI gene plays an essential role in the formation of the thiocarboxylate because ThiS isolated from a thiI(-) strain does not contain this modification. ThiF catalyzes the adenylation by ATP of the carboxyl-terminal glycine of ThiS. This reaction is likely to be involved in the activation of ThiS for sulfur transfer from cysteine or from a cysteine-derived sulfur donor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 14:17:36