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Titolo:
COLOCALIZATION OF CALCIUM-MODULATING CYCLOPHILIN LIGAND WITH INTRACELLULAR CALCIUM POOLS
Autore:
HOLLOWAY MP; BRAM RJ;
Indirizzi:
ST JUDE CHILDRENS HOSP,DEPT EXPT ONCOL,332 N LAUDERDALE ST MEMPHIS TN38105 ST JUDE CHILDRENS HOSP,DEPT EXPT ONCOL MEMPHIS TN 38105 ST JUDE CHILDRENS HOSP,DEPT HEMATOL ONCOL MEMPHIS TN 38105 UNIV TENNESSEE,DEPT PEDIAT MEMPHIS TN 38105
Titolo Testata:
The Journal of biological chemistry
fascicolo: 26, volume: 273, anno: 1998,
pagine: 16346 - 16350
SICI:
0021-9258(1998)273:26<16346:COCCLW>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOMA CELLS; INTEGRAL MEMBRANE-PROTEINS; INTERNAL CA2+ RELEASE; BINDING-PROTEIN; ENDOPLASMIC-RETICULUM; TRITON X-114; CALRETICULIN; INFLUX; IDENTIFICATION; THAPSIGARGIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
M.P. Holloway e R.J. Bram, "COLOCALIZATION OF CALCIUM-MODULATING CYCLOPHILIN LIGAND WITH INTRACELLULAR CALCIUM POOLS", The Journal of biological chemistry, 273(26), 1998, pp. 16346-16350

Abstract

The calcium-modulating cyclophilin ligand (CAML) protein activates Ca2+ influx signaling when overexpressed in Jurkat T cells. Although CAML appears to directly participate in Ca2+-dependent signaling initiated by the transmembrane activator and CAML interactor cell surface receptor, its mechanism of action is unknown. To address this issue, we have determined its membrane topology, subcellular localization, and ability to mobilize intracellular Ca2+ pools. Fractionation of cell extracts on discontinuous sucrose gradients and indirect immunofluorescenceindicate that CAML colocalizes with sarcoplasmic/endoplasmic reticulum calcium/ATPase-2 and calreticulin at membrane-bound cytosolic vesicles. Limited trypsin digests indicate that the hydrophilic NH2-terminaldomain of CAML is directed toward the cytoplasm. Functionally, CAML overexpression was shown to deplete thapsigargin-sensitive intracellular Ca2+ pools. These data suggest that CAML may initiate Ca2+ signalingthrough activation of a capacitative Ca2+ influx pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/08/20 alle ore 14:37:38