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Titolo:
PAXILLINE-NEGATIVE MUTANTS OF PENICILLIUM-PAXILLI GENERATED BY HETEROLOGOUS AND HOMOLOGOUS PLASMID INTEGRATION
Autore:
YOUNG C; ITOH Y; JOHNSON R; GARTHWAITE I; MILES CO; MUNDAYFINCH SC; SCOTT B;
Indirizzi:
MASSEY UNIV,INST MOL BIOSCI PALMERSTON NORTH NEW ZEALAND MASSEY UNIV,INST MOL BIOSCI PALMERSTON NORTH NEW ZEALAND SHINSHU UNIV,DEPT BIOL NAGANO 390 JAPAN AGRES,RUAKURA AGR RES CTR HAMILTON NEW ZEALAND
Titolo Testata:
Current genetics
fascicolo: 5, volume: 33, anno: 1998,
pagine: 368 - 377
SICI:
0172-8083(1998)33:5<368:PMOPGB>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYGROMYCIN-B RESISTANCE; ASPERGILLUS-NIDULANS; PERENNIAL RYEGRASS; NEUROSPORA-CRASSA; ESCHERICHIA-COLI; LOLIUM-PERENNE; TRANSFORMATION; DNA; BIOSYNTHESIS; SEQUENCES;
Keywords:
PENICILLIUM PAXILLI; PAXILLINE MUTANTS; PLASMID TAGGING; HOMOLOGOUS RECOMBINATION; DELETION MUTATIONS; PAN7-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
C. Young et al., "PAXILLINE-NEGATIVE MUTANTS OF PENICILLIUM-PAXILLI GENERATED BY HETEROLOGOUS AND HOMOLOGOUS PLASMID INTEGRATION", Current genetics, 33(5), 1998, pp. 368-377

Abstract

Using a monoclonal antibody based ELISA, 600 pAN7-1 plasmid-tagged mutants of Penicillium paxilli were screened for paxilline accumulation and one paxilline-negative mutant, YI-20, was identified. A molecular analysis of this mutant showed that pAN7-1 was inserted at a single site but was present as 4-6 copies arranged in a head-to-tail tandem array. Rescue of flanking sequences and analysis of the corresponding genomic region revealed that YI-20 has an extensive deletion at the site of pAN7-1 integration. Probing of a CHEF gel with the same sequences showed that associated with the deletion is a rearrangement of chromosome Va. Targeted gene disruption of wild-type sequences adjacent to thesite where pAN7-1 inserted, resulted in the generation of two additional paxilline-negative mutants; both were single crossovers with deletions extending outside the region mapped. Neither of these new mutantshad a rearrangement of chromosome Va, suggesting that deletion of genes on this chromosome is responsible for the paxilline-negative phenotype. Telomeric fingerprinting of genomic digests of P. paxilli, combined with pulsed-field gel electrophoresis of chromosomal DNA, established that there are a minimum of eight chromosomes in this fungus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 03:24:20