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Titolo:
OVER-EXPRESSION OF UROKINASE RECEPTOR IN HUMAN EPIDERMOID-CARCINOMA CELL-LINE (HEP3) INCREASES TUMORIGENICITY ON CHORIOALLANTOIC MEMBRANE AND IN SEVERE-COMBINED-IMMUNODEFICIENT MICE
Autore:
LYU MA; CHOI YK; PARK BN; PARK BJ; KIM BJ; PARK IK; HYUN BH; KOOK YH;
Indirizzi:
SEOUL NATL UNIV,COLL MED,CTR CANC RES,CHONGNO GU,28 YONGON DONG SEOUL110799 SOUTH KOREA SEOUL NATL UNIV,COLL MED,CTR CANC RES,CHONGNO GU SEOUL 110799 SOUTH KOREA SEOUL NATL UNIV,COLL MED,DEPT MICROBIOL SEOUL 110799 SOUTH KOREA KIST,KOREA RES INST BIOSCI & BIOTECHNOL,GENET RESOURCES CTR TAEJON SOUTH KOREA SEOUL NATL UNIV,COLL MED,DEPT PREVENT MED SEOUL SOUTH KOREA
Titolo Testata:
International journal of cancer
fascicolo: 2, volume: 77, anno: 1998,
pagine: 257 - 263
SICI:
0020-7136(1998)77:2<257:OOURIH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR; INVASION; CANCER; INHIBITION; MODULATION; SURFACE; GROWTH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Lyu et al., "OVER-EXPRESSION OF UROKINASE RECEPTOR IN HUMAN EPIDERMOID-CARCINOMA CELL-LINE (HEP3) INCREASES TUMORIGENICITY ON CHORIOALLANTOIC MEMBRANE AND IN SEVERE-COMBINED-IMMUNODEFICIENT MICE", International journal of cancer, 77(2), 1998, pp. 257-263

Abstract

Using chorio-allantoic membranes (CAMs) of chick embryos and severe-combined-immunodeficient (SCID) mice, we investigated the effects of urokinase-type plasminogen-activator receptor (u-PAR) over-expression onthe process of invasion and tumorigenicity. By the transfection of u-PAR cDNA, 3 u-PAR-over-expressing clones expressing 1.6- to 4.6-fold more u-PAR mRNA than parent cells were obtained from a human epidermoid-carcinoma cell line, HEp3, that expresses urokinase-type plasminogen activator (U-PA) and u-PAR. All the u-PAR-over-expressing clones showed greater invasiveness (13 to 29%) than that of parent HEp3 cells on CAMs. Immunohistochemistry revealed densely stained u-PAR-positive cells near the margin of the tumor, where a u-PAR-over-expressing clone, designated SM-3, was invading thickened fibrous tissue on CAMs. Three u-PAR-overexpressing clones formed larger tumors (>40 mm(3)) than did parent HEp3 cells on CAMs. Moreover, when the u-PAR-overexpressing clone (SM-3) was injected s.c. into the back of the SCID mice it produced a larger tumor volume than the control(HEp3) and down-regulated (AS-2)clones and significantly shortened the survival of SCID mice. These results demonstrate that increased u-PAR expression is an important factor in determining the malignant phenotype that makes cancer cells more invasive and tumorigenic. (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 14:24:51