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Titolo:
PROGRAMMED CELL-DEATH CONTRIBUTES TO POSTNATAL LUNG DEVELOPMENT
Autore:
SCHITTNY JC; DJONOV V; FINE L; BURRI PH;
Indirizzi:
UNIV BERN,DEPT DEV BIOL,INST ANAT CH-3012 BERN SWITZERLAND BOSTON UNIV,SCH MED,CTR PULM BOSTON MA 02118
Titolo Testata:
American journal of respiratory cell and molecular biology
fascicolo: 6, volume: 18, anno: 1998,
pagine: 786 - 793
SICI:
1044-1549(1998)18:6<786:PCCTPL>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT LUNG; DNA FRAGMENTATION; APOPTOSIS; GROWTH; INJURY; ALVEOLI; FETAL; PNEUMOCYTES; MORPHOLOGY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Schittny et al., "PROGRAMMED CELL-DEATH CONTRIBUTES TO POSTNATAL LUNG DEVELOPMENT", American journal of respiratory cell and molecular biology, 18(6), 1998, pp. 786-793

Abstract

The rat lune undergoes the phase of maturation of the alveolar septa and of the parenchymal microvascular network mainly during the third postnatal week. Speculating that programmed cell death may contribute to the thinning of the alveolar septa, we searched for the presence of DNA fragmentation in rat lungs between postnatal days 6 and 36 using the TUNEL procedure, The number of positive nuclei was compared at different days. We observed an 8-fold increase of programmed cell death toward the end of the third week as compared to the days before and after this time point. The precise timing of the appearance of the peak depended on the size of the litter, Double-labeling for DNA fragmentation (TUNEL) and for type I and type II epithelial cells (antibodies E11 and MNF-116), as well as morphologic studies at electron microscopic level, revealed that during the peak of programmed cell death mainly fibroblasts and type II epithelial cells were dying. While both dying cell types were TUNEL-positive. nuclear fragments and apoptotic bodies were exclusively observed in the dying fibroblasts. We conclude that programmed cell death is involved in the structural maturation of the lung by reducing the number of fibroblasts and type II epithelial cells in the third postnatal week, We observed that the dying fibroblasts are cleared by neighboring fibroblasts in a later stage of apoptosis, and we hypothesize that type II epithelial cells are cleared by alveolarmacrophages in early stages of the programmed cell death process.

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Documento generato il 28/09/20 alle ore 14:23:50