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Titolo:
TRANSDUCTION OF THE SKBR3 BREAST-CARCINOMA CELL-LINE WITH THE HOXB7 GENE INDUCES BFGF EXPRESSION, INCREASES CELL-PROLIFERATION AND REDUCES GROWTH-FACTOR DEPENDENCE
Autore:
CARE A; SILVANI A; MECCIA E; MATTIA G; PESCHLE C; COLOMBO MP;
Indirizzi:
IST NAZL STUDIO & CURA TUMORI,DIV EXPT ONCOL D,VIA VENEZIAN 1 I-20133MILAN ITALY IST NAZL STUDIO & CURA TUMORI,DIV EXPT ONCOL D I-20133 MILAN ITALY IST SUPER SANITA,DEPT HEMATOL & ONCOL I-00161 ROME ITALY
Titolo Testata:
Oncogene
fascicolo: 25, volume: 16, anno: 1998,
pagine: 3285 - 3289
SICI:
0950-9232(1998)16:25<3285:TOTSBC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMEOBOX GENES; TUMORS; MICE; LEUKEMIA; PROMOTER; CANCER;
Keywords:
BFGF; HOMEOBOX GENE, SKBR3 CELL LINE; RETROVIRAL VECTOR; INTRACRINE LOOP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
A. Care et al., "TRANSDUCTION OF THE SKBR3 BREAST-CARCINOMA CELL-LINE WITH THE HOXB7 GENE INDUCES BFGF EXPRESSION, INCREASES CELL-PROLIFERATION AND REDUCES GROWTH-FACTOR DEPENDENCE", Oncogene, 16(25), 1998, pp. 3285-3289

Abstract

Several melanomas, carcinomas, glioblastomas and leukemias showed coordinated expression of HOXB7 and bFGF with exception of the SkBr3 mammary carcinoma that was negative for both. Transduction of HOXB7 gene into SkBr3 cells, induced bFGF expression, increased growth rate, independence from serum withdrawal and ability to form colonies in semisolid medium. ELISA assay showed that most of bFGF was associated to cell lysate when cells were cultured at 1% serum whereas in cells kept to 10% serum bFGF was detected both within cell lysate or secreted into cell supernatants. Antisense oligos to bFGF inhibited the growth of cells cultured in 1%, indicating that beside the possible activation of additional genes other than bFGF by HOXB7 transduction, only bFGF induction accounts for the observed results. Moreover, since inhibition of cell proliferation occurred in cells kept in 1% but not 10% serum, a bFGF intracrine loop appears operative in serum starved SkBr3/HOXB7 cells. Also, these results further indicate bFGF as target of HOXB7.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:45:54