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Titolo:
A 2ND-GENERATION ENZYME-IMMUNOASSAY FOR DETECTION OF ANTIBODIES TO IFN-ALPHA
Autore:
PALLERONI AV; SPECIAN A; AGLIONE A; DVOROZNIAK MT; RAMAN J;
Indirizzi:
HOFFMANN LA ROCHE INC,ROCHE RES CTR NUTLEY NJ 07110
Titolo Testata:
Journal of interferon & cytokine research
fascicolo: 6, volume: 18, anno: 1998,
pagine: 357 - 360
SICI:
1079-9907(1998)18:6<357:A2EFDO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFERON; SERUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
A.V. Palleroni et al., "A 2ND-GENERATION ENZYME-IMMUNOASSAY FOR DETECTION OF ANTIBODIES TO IFN-ALPHA", Journal of interferon & cytokine research, 18(6), 1998, pp. 357-360

Abstract

We have developed a solid-phase enzyme-linked immunoassay (EIA) for detecting antibodies to interferon-alpha 2 (IFN-alpha 2) in serum or plasma. In this assay, based on the sandwich principle, the capture antigen, IFN-alpha 2, is covalently bound to the wells in 96-well plates, This novel procedure offers considerable advantages over the antigen binding by passive adsorption used in most previous EIA, Specific antibodies present in clinical specimens bind to the anchored antigen and are detected by adding peroxidase-labeled IFN-alpha 2 and a peroxidase substrate mixture, The resultant color is a function of the concentration of antibody in the sample, The assay has proved to be convenient, precise, and reproducible and can detect as little as 1-5 ng/ml of specific antibody IgG.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 15:52:47