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Titolo:
SYNTHESIS, CHARACTERIZATION, AND SWEETNESS-SUPPRESSING ACTIVITIES OF GURMARIN ANALOGS MISSING ONE DISULFIDE BOND
Autore:
OTA M; SHIMIZU Y; TONOSAKI K; ARIYOSHI Y;
Indirizzi:
AJINOMOTO CO INC,CENT RES LABS,KAWASAKI KU,1-1 SUZUKI CHO KAWASAKI KANAGAWA 210 JAPAN GIFU UNIV,FAC AGR,DEPT VET PHYSIOL GIFU 50111 JAPAN
Titolo Testata:
Biopolymers
fascicolo: 2, volume: 46, anno: 1998,
pagine: 65 - 73
SICI:
0006-3525(1998)46:2<65:SCASAO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYPEPTIDE GURMARIN; GYMNEMA-SYLVESTRE; PEPTIDE GURMARIN; TASTE RESPONSES; NERVE RESPONSES; INHIBITION; STIMULI; LEAVES; SUGARS; MICE;
Keywords:
GURMARIN; GURMARIN ANALOGS; SWEETNESS SUPPRESSION; SYNTHETIC PEPTIDE; CHORDA TYMPANI NERVE; GURMARIN MISSING DISULFIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
M. Ota et al., "SYNTHESIS, CHARACTERIZATION, AND SWEETNESS-SUPPRESSING ACTIVITIES OF GURMARIN ANALOGS MISSING ONE DISULFIDE BOND", Biopolymers, 46(2), 1998, pp. 65-73

Abstract

The sweetness-suppressing polypeptide gurmarin isolated from Gymnema sylvestre consists of 35 amino acid residues and includes three intramolecular disulfide bonds. The roles of the three disulfide bonds were investigated by replacing each with two alanine residues by solid-phase synthesis. Nine analogues of [Ala(3,18)]gurmarin, [Ala(10,23)]gurmarin, and [Ala(17,33)]gurmarin were obtained. Three analogues had nativedisulfide bonds, while the other six had non-native disulfide bonds. The three analogues with native disulfide bonds suppressed the response to sucrose, but not those to glucose, fructose, saccharin, or glycine in rats. In contrast, the six analogues with non-native disulfide bonds did not suppress the responses to any of these sweeteners. These results suggest that the native disulfide bonds of gurmarin are necessary for interaction with the receptor protein, and that the sucrose-specific receptor sire is present in rats. (C) 1998 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:01:40