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Titolo: T-CELL ACTIVATION-INDUCED BY NOVEL GAIN-OF-FUNCTION MUTANTS OF SYK AND ZAP-70
Autore: ZEITLMANN L; KNORR T; KNOLL M; ROMEO C; SIRIM P; KOLANUS W;
- Indirizzi:
- UNIV MUNICH,MOL BIOL LAB,GENZENTRUM,FEODOR LYNEN STR 25 D-81377 MUNICH GERMANY UNIV MUNICH,MOL BIOL LAB,GENZENTRUM D-81377 MUNICH GERMANY NIEHS,NIH RES TRIANGLE PK NC 27709
- Titolo Testata:
- The Journal of biological chemistry
fascicolo: 25,
volume: 273,
anno: 1998,
pagine: 15445 - 15452
- SICI:
- 0021-9258(1998)273:25<15445:TABNGM>2.0.ZU;2-O
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- PROTEIN-TYROSINE KINASE; RECEPTOR ZETA-CHAIN; DOMAIN-CONTAINING PROTEINS; TANDEM SH2 DOMAINS; ANTIGEN RECEPTOR; SIGNAL-TRANSDUCTION; EXPRESSION SYSTEM; BINDING; PHOSPHORYLATION; TCR;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Citazioni:
- 48
- Recensione:
- Indirizzi per estratti:
-
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- Citazione:
- L. Zeitlmann et al., "T-CELL ACTIVATION-INDUCED BY NOVEL GAIN-OF-FUNCTION MUTANTS OF SYK AND ZAP-70", The Journal of biological chemistry, 273(25), 1998, pp. 15445-15452
Abstract
The Syk family tyrosine kinases play a crucial role in antigen receptor-mediated signal transduction, but their regulation and cellular targets remain incompletely defined. Following receptor engagement, phosphorylation of tyrosine residues within ZAP-70 and Syk is thought to control both kinase activity and recruitment of modulatory factors. We report here the characterization of novel mutants of ZAP-70 and Syk, inwhich conserved C-terminal tyrosine residues have been replaced by phenylalanines (ZAP YF-C, Syk YF-C), Both mutant kinases display a prominent gain-of-function phenotype in Jurkat T cells, as demonstrated by lymphokine promoter activation, tyrosine phosphorylation of potential targets in vivo, and elevated intracellular calcium mobilization. While the presence of p56-Lck was required for ZAP YF-C-induced signaling,Syk YF-C showed enhanced functional activity in Lck-deficient JCaM1 Jurkat cells. Our results implicate the C terminus of Syk family kinases as an important regulatory region modulating T cell activation.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:55:14