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Titolo:
MOLECULAR MECHANISM OF HUMAN CD38 GENE-EXPRESSION BY RETINOIC ACID - IDENTIFICATION OF RETINOIC ACID RESPONSE ELEMENT IN THE FIRST INTRON
Autore:
KISHIMOTO H; HOSHINO S; OHORI M; KONTANI K; NISHINA H; SUZAWA M; KATO S; KATADA T;
Indirizzi:
UNIV TOKYO,GRAD SCH PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,GRAD SCH PHARMACEUT SCI,DEPT PHYSIOL CHEM,BUNKYO KU TOKYO 113 JAPAN UNIV TOKYO,INST MOL & CELLULAR BIOSCI,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 25, volume: 273, anno: 1998,
pagine: 15429 - 15434
SICI:
0021-9258(1998)273:25<15429:MMOHCG>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYL CYCLASE; LYMPHOCYTE ANTIGEN CD38; NAD GLYCOHYDROLASE; MONOCLONAL-ANTIBODY; LEUKEMIA-CELLS; MYELOID CELLS; B-CELLS; SURFACE; DIFFERENTIATION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
H. Kishimoto et al., "MOLECULAR MECHANISM OF HUMAN CD38 GENE-EXPRESSION BY RETINOIC ACID - IDENTIFICATION OF RETINOIC ACID RESPONSE ELEMENT IN THE FIRST INTRON", The Journal of biological chemistry, 273(25), 1998, pp. 15429-15434

Abstract

CD38 is a nonlineage-restricted type II transmembrane glycoprotein possessing ecto-NAD(+) glycohydrolase activity. Because of its unique expression pattern in lymphocyte differentiation, it appears to functionas an immunoregulatory molecule. We previously reported that CD38 wasspecifically induced by all-trans-retinoic acid (RA) in human promyelocytic leukemia HL-60 cells. Here we studied the molecular mechanism of the RA-dependent induction of human CD38, The expression of CD38 mRNA by RA appeared to be caused by the transcriptional stimulation of the gene, since it was blocked by an RNA synthesis inhibitor, but not bya protein synthesis inhibitor. In search of the RA response element (RARE) possibly present in human CD38 gene promoter, we isolated and sequenced the genomic DNA covering the 5'-flanking region, exon I, and partial intron 1. Transient transfection experiments revealed that the responsiveness to RA was conferred through an RARE consisting of two direct repeat TGACCT-like hexamer motifs with a 5-nucleotide spacer, which was located in the first intron rather than the 5'-flanking regionof the CD38 gene. This RARE interacted with heterodimer composed of RA receptor and retinoid X receptor in vitro. Thus, the RA-induced expression of the human CD38 gene was demonstrated to be mediated through the RARE located in the first intron.

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Documento generato il 04/12/20 alle ore 17:51:49