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Titolo:
IMMUNE MODULATION OF HUMAN B-LYMPHOCYTES BY GENE-TRANSFER WITH RECOMBINANT EPSTEIN-BARR-VIRUS AMPLICONS
Autore:
WANG F; SELDIN DC; ANNIS B; TROCHA A; JOHNSON RP;
Indirizzi:
HARVARD UNIV,BRIGHAM & WOMENS HOSP,SCH MED,DEPT MED,181 LONGWOOD AVE BOSTON MA 02115 BOSTON UNIV,MED CTR,DEPT MED BOSTON MA 02118 BOSTON UNIV,MED CTR,DEPT MICROBIOL BOSTON MA 02118 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,PARTNERS AIDS RES CTR CHARLESTOWN MA 02129 HARVARD UNIV,SCH MED,MASSACHUSETTS GEN HOSP,INFECT DIS UNIT CHARLESTOWN MA 02129 HARVARD UNIV,SCH MED,NEW ENGLAND REG PRIMATE RES CTR,DIV IMMUNOL SOUTHBOROUGH MA 01172
Titolo Testata:
Journal of virological methods
fascicolo: 1, volume: 72, anno: 1998,
pagine: 81 - 93
SICI:
0166-0934(1998)72:1<81:IMOHBB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONSERVED REGION; EBV RECOMBINANTS; CELLS; TYPE-1; INTERLEUKIN-4; DELETION; INVITRO; DNA; TRANSFORMATION; IDENTIFICATION;
Keywords:
GENE TRANSFER; EPSTEIN-BARR VIRUS; ANTIGEN PRESENTATION; CYTOTOXIC T CELL; CYTOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
F. Wang et al., "IMMUNE MODULATION OF HUMAN B-LYMPHOCYTES BY GENE-TRANSFER WITH RECOMBINANT EPSTEIN-BARR-VIRUS AMPLICONS", Journal of virological methods, 72(1), 1998, pp. 81-93

Abstract

We described previously a novel mode of gene transfer by infection ofhuman B lymphocytes with recombinant Epstein-Barr virus (EBV) amplicons. This system was explored for its potential use in expressing various recombinant genes, including the cytokine IL-4, the HIV envelope glycoprotein (gp120) and a suicide and gag gene. Recombinant genes were present as multiple copy episomes and stable, high level recombinant gene expression could be detected by antigenic and functional assays. Amplicon-infected B cells secreted high levels of recombinant cytokine and efficiently presented recombinant antigens through classes I and IT MHC-restricted antigen processing pathways. Thus, recombinant EBV amplicons can be used to express components of the immune system or heterologous genes for immune recognition in human B cells. Combining genetransfer with EBV infection may provide unique advantages for in vitro and in vivo gene transfer. (C) 1998 Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:26:18