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Titolo:
SUCCINOGLYCAN PRODUCTION BY AGROBACTERIUM-TUMEFACIENS
Autore:
STREDANSKY M; CONTI E; BERTOCCHI C; MATULOVA M; ZANETTI F;
Indirizzi:
POLY TECH SOC COOP ARL,AREA SCI PK,PADRICIANO 99 I-34012 TRIESTE ITALY SLOVAK ACAD SCI,INST CHEM BRATISLAVA SLOVAKIA
Titolo Testata:
Journal of fermentation and bioengineering
fascicolo: 4, volume: 85, anno: 1998,
pagine: 398 - 403
SICI:
0922-338X(1998)85:4<398:SPBA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-13 NMR-SPECTRA; XANTHOMONAS-CAMPESTRIS; EXOPOLYSACCHARIDE; GROWTH; RADIOBACTER; BIOSYNTHESIS; ASSIGNMENT; SUCCINATE; PHOSPHATE; LOCATION;
Keywords:
AGROBACTERIUM TUMEFACIENS; SUCCINOGLYCAN; FERMENTATION; MEDIUM COMPOSITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
M. Stredansky et al., "SUCCINOGLYCAN PRODUCTION BY AGROBACTERIUM-TUMEFACIENS", Journal of fermentation and bioengineering, 85(4), 1998, pp. 398-403

Abstract

A strain of Agrobacterium tumefaciens isolated from soil was cultivated under various conditions in shake flasks to study exopolysaccharides (EPS) production. NMR analysis revealed that the EPS obtained was a succinoglycan-like polymer. Optimal yields of EPS were obtained using sucrose and lysine as the carbon and nitrogen sources, respectively. Supplementation of the medium with various chemicals resulted in a moreor less marked effect on the polymer yield and properties: in particular, high phosphate levels and non-ionic surfactants led to the production of polymers of different molecular sizes in yields up to 13.7 g/l. Oxygen availability also affected the polymer yield and quality. Thechemical structure was substantially unaffected by the various fermentation conditions tested. A fermentation carried out in a laboratory-scale fermenter yielded 9.6 g/l succinoglycan from 15 g/l sucrose in the basic medium without further supplements.

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Documento generato il 13/07/20 alle ore 07:48:27