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Titolo:
ANALYSES OF CDNA AND RECOMBINANT PROTEIN FOR A POTENT VASOACTIVE PROTEIN IN SALIVA OF A BLOOD-FEEDING BLACK FLY, SIMULIUM-VITTATUM
Autore:
CUPP MS; RIBEIRO JMC; CHAMPAGNE DE; CUPP EW;
Indirizzi:
UNIV ARIZONA,DEPT ENTOMOL TUCSON AZ 85721 AUBURN UNIV,DEPT ENTOMOL AUBURN AL 36849 NIAID,PARASIT DIS LAB,NIH BETHESDA MD 20892 UNIV GEORGIA,DEPT ENTOMOL ATHENS GA 30602
Titolo Testata:
Journal of Experimental Biology
fascicolo: 10, volume: 201, anno: 1998,
pagine: 1553 - 1561
SICI:
0022-0949(1998)201:10<1553:AOCARP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LUTZOMYIA-LONGIPALPIS; FINDING STRATEGY; GLAND EXTRACT; AEDES-AEGYPTI; FLIES DIPTERA; RESPONSES; MODULATION; ARTHROPODS; MAXADILAN;
Keywords:
ERYTHEMA; VASOACTIVE PROTEIN; SIMULIUM VITTATUM; BLACK FLY; SALIVARY GLAND; CDNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
M.S. Cupp et al., "ANALYSES OF CDNA AND RECOMBINANT PROTEIN FOR A POTENT VASOACTIVE PROTEIN IN SALIVA OF A BLOOD-FEEDING BLACK FLY, SIMULIUM-VITTATUM", Journal of Experimental Biology, 201(10), 1998, pp. 1553-1561

Abstract

A cDNA was cloned from the salivary glands of a blood-feeding black fly Simulium vittatum. The encoded protein has been given the name Simulium vittatum erythema protein or SVEP, because of its ability to increase blood perfusion in skin capillaries, resulting in the well-characterized erythema of black fly bites. The full-length cDNA contains 548base pairs which encode 152 amino acid residues of the nascent protein. Post-translational processing produces a mature, secreted protein of 133 residues with a molecular mass of 15.4 kDa. Recombinant SVEP (rSVEP) was produced in a baculovirus expression system and purified by aone-step reversed-phase HPLC procedure. Analyses of physical properties and biological potency demonstrated fidelity of rSVEP to the nativeprotein. Recombinant SVEP relaxed rabbit aorta preparations when preconstricted with 2 mu mol l(-1) phenylephrine or 25 mmol l(-1) K+ but not with 60 mmol l(-1) K+. Further, the rSVEP-induced relaxation response of phenylephrine-constricted aorta was inhibited by glibenclamide (10 mu mol l(-1)), suggesting that at least part of its action to relaxsmooth muscle may result from the opening of ATP-dependent K(+)channels. SVEP is a novel salivary-gland-derived vasoactive protein that maybe essential for blood feeding by black flies and could potentially enhance transmissionof filarial parasites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:01:12