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Titolo:
INTERPHASE CYTOGENETICS AND COMPETITIVE RT-PCR FOR RESIDUAL DISEASE MONITORING IN PATIENTS WITH CHRONIC MYELOID-LEUKEMIA DURING INTERFERON-ALPHA THERAPY
Autore:
TCHIRKOV A; GIOLLANT M; TAVERNIER F; BRIANCON G; TOURNILHAC O; KWIATKOWSKI F; PHILIPPE P; CHOUFI B; DEMEOCQ F; TRAVADE P; MALET P;
Indirizzi:
FAC MED,LAB CYTOGENET MED,PL HENRI DUNANT,BP 38 F-63001 CLERMONT FERRA FRANCE CHU CLERMONT FERRAND,HOTEL DIEU,SERV HEMATOL CLIN CLERMONT FERRA FRANCE CTR JEAN PERRIN,SERV STAT & COMMUN MED CLERMONT FERRA FRANCE CHU CLERMONT FERRAND,HOTEL DIEU,SERV MED INTERNE CLERMONT FERRA FRANCE CTR JEAN PERRIN,UNITE TRANSPLANTAT MEDULLAIRE CLERMONT FERRA FRANCE CHU CLERMONT FERRAND,HOTEL DIEU,SERV PEDIAT CLERMONT FERRA FRANCE
Titolo Testata:
British Journal of Haematology
fascicolo: 3, volume: 101, anno: 1998,
pagine: 552 - 557
SICI:
0007-1048(1998)101:3<552:ICACRF>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC MYELOGENOUS LEUKEMIA; IN-SITU HYBRIDIZATION; ACUTE LYMPHOBLASTIC-LEUKEMIA; POLYMERASE CHAIN-REACTION; BCR-ABL TRANSCRIPTS; BONE-MARROW; BLOOD; QUANTIFICATION; CELLS;
Keywords:
CML; RESPONSE TO INTERFERON-ALPHA; BCR-ABL GENE; INTERPHASE FISH; COMPETITIVE RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
A. Tchirkov et al., "INTERPHASE CYTOGENETICS AND COMPETITIVE RT-PCR FOR RESIDUAL DISEASE MONITORING IN PATIENTS WITH CHRONIC MYELOID-LEUKEMIA DURING INTERFERON-ALPHA THERAPY", British Journal of Haematology, 101(3), 1998, pp. 552-557

Abstract

There is a need for fast and sensitive methods to evaluate the response of patients with chronic myeloid leukaemia (CML) to interferon-alpha (IFN-alpha) therapy to complement cytogenetic analysis of Philadelphia (Ph) chromosome-positive metaphases. We have used interphase FISH (fluorescence in situ hybridization) and competitive RT-PCR (reverse transcriptase-polymerase chain reaction) techniques for detection of BCR-ABL-positive cells to measure suppression of leukaemic clone in a series of 51 follow-up samples from 24 CML patients undergoing IFN-alpha treatment. Interphase FISH analysis of the malignant clone in bone marrow using BCR and ABL probes was found to be highly correlated to conventional G-banding metaphase examination (r = 0.98). RT-PCR quantification of BCR-ABL mRNA transcripts in blood also showed a high degree ofconcordance with the proportion of Ph-positive metaphases (r = 0.93). In addition, the degree of cytogenetic response did not influence theequivalence between karyotype analysis and molecular methods. We concluded that interphase FISH and competitive RT-PCR provide reliable information on residual tumour burden and response to IFN-alpha in CML patients. These molecular methods may significantly improve the efficiency of residual disease monitoring during IFN-alpha therapy of CML.

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Documento generato il 30/11/20 alle ore 06:37:33