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Titolo:
PREDICTION OF FUNCTIONAL RESIDUES IN WATER CHANNELS AND RELATED PROTEINS
Autore:
FROGER A; TALLUR B; THOMAS D; DELAMARCHE C;
Indirizzi:
UNIV RENNES 1,UPRES A CNRS 6026,EQUIPE CANAUX & RECEPTEURS MEMBRANAIRES,BATIMENT 13 F-35042 RENNES FRANCE UNIV RENNES 1,UPRES A CNRS 6026,EQUIPE CANAUX & RECEPTEURS MEMBRANAIRES F-35042 RENNES FRANCE INST RECH INFORMAT & SYST ALEATOIRES,IRISA F-35042 RENNES FRANCE
Titolo Testata:
Protein science
fascicolo: 6, volume: 7, anno: 1998,
pagine: 1458 - 1468
SICI:
0961-8368(1998)7:6<1458:POFRIW>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR INTRINSIC PROTEIN; MIP FAMILY; ESCHERICHIA-COLI; MEMBRANE-PROTEIN; XENOPUS-OOCYTES; SEQUENCE ALIGNMENTS; MOLECULAR-CLONING; AQUAPORIN CHIP; TRANSPORT; EVOLUTION;
Keywords:
AQUAPORIN; CORRESPONDENCE ANALYSIS; GLYCEROL FACILITATOR; MIP FAMILY; MULTIPLE SEQUENCE ALIGNMENT; PROTEIN FUNCTION PREDICTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
A. Froger et al., "PREDICTION OF FUNCTIONAL RESIDUES IN WATER CHANNELS AND RELATED PROTEINS", Protein science, 7(6), 1998, pp. 1458-1468

Abstract

In this paper, we present an updated classification of the ubiquitousMIP (Major Intrinsic Protein) family proteins, including 153 fully orpartially sequenced members available in public databases. Presently,about 30 of these proteins have been functionally characterized, exhibiting essentially two distinct types of channel properties: (1) specific water transport by the aquaporins, and (2) small neutral solutes transport, such as glycerol by the glycerol facilitators. Sequence alignments were used to predict amino acids and motifs discriminant in channel specificity. The protein sequences were also analyzed using statistical tools (comparisons of means and correspondence analysis). Five key positions were clearly identified where the residues are specific for each functional subgroup and exhibit high dissimilar physico-chemical properties. Moreover, we have found that the putative channels forsmall neutral solutes clearly differ from the aquaporins by the aminoacid content and the length of predicted loop regions, suggesting a substrate filter function for these loops. From these results, we propose a signature pattern for water transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:08:43